CT domain of CCN2/CTGF directly interacts with fibronectin and enhances cell adhesion of chondrocytes through integrin α5β1

Mitsuhiro Hoshijima, Takako Hattori, Miho Inoue, Daisuke Araki, Hiroshi Hanagata, Akira Miyauchi, Masaharu Takigawa

Research output: Contribution to journalArticlepeer-review

99 Citations (Scopus)

Abstract

Searching for CCN family protein 2/connective tissue growth factor (CCN2/CTGF) interactive proteins by yeast-two-hybrid screening, we identified fibronectin 1 gene product as a major binding partner of CCN2/CTGF in the chondrosarcoma-derived chondrocytic cell line HCS-2/8. Only the CT domain of CCN2/CTGF bound directly to fibronectin (FN). CCN2/CTGF and its CT domain enhanced the adhesion of HCS-2/8 cells to FN in a dose-dependent manner. The CCN2/CTGF-enhancing effect on cell adhesion to FN was abolished by a blocking antibody against α5β1 integrin (α5β1), but not by one against anti-αvβ3 integrin. These findings suggest for the first time that CCN2/CTGF enhances chondrocyte adhesion to FN through direct interaction of its C-terminal CT domain with FN, and that α5β1 is involved in this adhesion.

Original languageEnglish
Pages (from-to)1376-1382
Number of pages7
JournalFEBS Letters
Volume580
Issue number5
DOIs
Publication statusPublished - Feb 20 2006

Keywords

  • Binding protein
  • CCN family
  • CCN family protein 2/connective tissue growth factor
  • Cell adhesion
  • Fibronectin
  • Integrin
  • α5β1
  • αvβ3

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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