Crystallographic and in Silico Analysis of the Sialoside-binding Characteristics of the Siglec Sialoadhesin

Nathan R. Zaccai, Andrew P. May, Robert C. Robinson, Leslie D. Burtnick, Paul R. Crocker, Reinhard Brossmer, Soerge Kelm, E. Yvonne Jones

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The Siglec family of receptors mediates cell-surface interactions through recognition of sialylated glycoconjugates. Previously reported structures of the N-terminal domain of the Siglec sialoadhesin (SnD1) in complex with various sialic acid analogs revealed the structural template for sialic acid binding. To characterize further the carbohydrate-binding properties, we have determined the crystal structures of SnD1 in the absence of ligand, and in complex with 2-benzyl-Neu5NPro and 2-benzyl-Neu5NAc. These structures reveal that SnD1 undergoes very few structural changes on ligand binding and detail how two novel classes of sialic acid analogs bind, one of which unexpectedly can induce Siglec dimerization. In conjunction with in silico analysis, this set of structures informs us about the design of putative ligands with enhanced binding affinities and specificities to different Siglecs, and provides data with which to test the effectiveness of different computational drug design protocols.

Original languageEnglish
Pages (from-to)1469-1479
Number of pages11
JournalJournal of Molecular Biology
Volume365
Issue number5
DOIs
Publication statusPublished - Feb 2 2007
Externally publishedYes

Fingerprint

Sialic Acid Binding Ig-like Lectin 1
Sialic Acid Binding Immunoglobulin-like Lectins
Computer Simulation
N-Acetylneuraminic Acid
Ligands
Glycoconjugates
Drug Design
Dimerization
Cell Communication
Carbohydrates

Keywords

  • GRID
  • sialic acid
  • sialoadhesin
  • Siglec
  • structure-based drug design

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Crystallographic and in Silico Analysis of the Sialoside-binding Characteristics of the Siglec Sialoadhesin. / Zaccai, Nathan R.; May, Andrew P.; Robinson, Robert C.; Burtnick, Leslie D.; Crocker, Paul R.; Brossmer, Reinhard; Kelm, Soerge; Jones, E. Yvonne.

In: Journal of Molecular Biology, Vol. 365, No. 5, 02.02.2007, p. 1469-1479.

Research output: Contribution to journalArticle

Zaccai, Nathan R. ; May, Andrew P. ; Robinson, Robert C. ; Burtnick, Leslie D. ; Crocker, Paul R. ; Brossmer, Reinhard ; Kelm, Soerge ; Jones, E. Yvonne. / Crystallographic and in Silico Analysis of the Sialoside-binding Characteristics of the Siglec Sialoadhesin. In: Journal of Molecular Biology. 2007 ; Vol. 365, No. 5. pp. 1469-1479.
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