Crystallographic analysis reveals Octamerization of viroplasm matrix protein P9-1 of rice black streaked dwarf virus

Fusamichi Akita, Akifumi Higashiura, Takumi Shimizu, Yingying Pu, Mamoru Suzuki, Tamaki Uehara-Ichiki, Takahide Sasaya, Shuji Kanamaru, Fumio Arisaka, Tomitake Tsukihara, Atsushi Nakagawa, Toshihiro Omura

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The P9-1 protein of Rice black streaked dwarf virus accumulates in viroplasm inclusions, which are structures that appear to play an important role in viral morphogenesis and are commonly found in viruses in the family Reoviridae. Crystallographic analysis of P9-1 revealed structural features that allow the protein to form dimers via hydrophobic interactions. Each dimer has carboxy-terminal regions, resembling arms, that extend to neighboring dimers, thereby uniting sets of four dimers via lateral hydrophobic interactions, to yield cylindrical octamers. The importance of these regions for the formation of viroplasm-like inclusions was confirmed by the absence of such inclusions when P9-1 was expressed without its carboxy-terminal arm. The octamers are vertically elongated cylinders resembling the structures formed by NSP2 of rotavirus, even though there are no significant similarities between the respective primary and secondary structures of the two proteins. Our results suggest that an octameric structure with an internal pore might be important for the functioning of the respective proteins in the events that occur in the viroplasm, which might include viral morphogenesis.

Original languageEnglish
Pages (from-to)746-756
Number of pages11
JournalJournal of Virology
Volume86
Issue number2
DOIs
Publication statusPublished - Jan 2012
Externally publishedYes

Fingerprint

Rice black streaked dwarf virus
Viruses
Hydrophobic and Hydrophilic Interactions
Morphogenesis
hydrophobic bonding
Reoviridae
morphogenesis
Secondary Protein Structure
Proteins
proteins
Rotavirus
viruses
viral inclusion bodies
Oryza

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Crystallographic analysis reveals Octamerization of viroplasm matrix protein P9-1 of rice black streaked dwarf virus. / Akita, Fusamichi; Higashiura, Akifumi; Shimizu, Takumi; Pu, Yingying; Suzuki, Mamoru; Uehara-Ichiki, Tamaki; Sasaya, Takahide; Kanamaru, Shuji; Arisaka, Fumio; Tsukihara, Tomitake; Nakagawa, Atsushi; Omura, Toshihiro.

In: Journal of Virology, Vol. 86, No. 2, 01.2012, p. 746-756.

Research output: Contribution to journalArticle

Akita, F, Higashiura, A, Shimizu, T, Pu, Y, Suzuki, M, Uehara-Ichiki, T, Sasaya, T, Kanamaru, S, Arisaka, F, Tsukihara, T, Nakagawa, A & Omura, T 2012, 'Crystallographic analysis reveals Octamerization of viroplasm matrix protein P9-1 of rice black streaked dwarf virus', Journal of Virology, vol. 86, no. 2, pp. 746-756. https://doi.org/10.1128/JVI.00826-11
Akita, Fusamichi ; Higashiura, Akifumi ; Shimizu, Takumi ; Pu, Yingying ; Suzuki, Mamoru ; Uehara-Ichiki, Tamaki ; Sasaya, Takahide ; Kanamaru, Shuji ; Arisaka, Fumio ; Tsukihara, Tomitake ; Nakagawa, Atsushi ; Omura, Toshihiro. / Crystallographic analysis reveals Octamerization of viroplasm matrix protein P9-1 of rice black streaked dwarf virus. In: Journal of Virology. 2012 ; Vol. 86, No. 2. pp. 746-756.
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