Crystallization and preliminary X-ray study of two crystal forms of Klebsiella oxytoca diol dehydratase-cyanocobalamin complex

Jun Masuda, Tetsuya Yamaguchi, Takamasa Tobimatsu, Tetsuo Toraya, Kyoko Suto, Naoki Shibata, Yukio Morimoto, Yoshiki Higuchi, Noritake Yasuoka

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Two crystal forms of Klebsiella oxytoca diol dehydratase complexed with cyanocobalamin have been obtained and preliminary crystallographic experiments have been performed. The crystals belong to two different space groups, depending on the crystallization conditions. One crystal (form I) belongs to space group P212121 with unit-cell parameters a = 76.2, b = 122.3, c = 209.6 Å, and diffracts to 2.2 Å resolution using an X-ray beam from a synchrotron radiation source. The other crystal (form II) belongs to space group P21 with unit-cell parameters a = 75.4, b = 132.7, c = 298.8 Å, β = 91.9°, and diffracts to 3.0 Å resolution. For the purpose of structure determination, a heavy-atom derivative search was carried out and some mercuric derivatives were found to be promising. Structure analysis by the multiple isomorphous replacement method is now under way.

Original languageEnglish
Pages (from-to)907-909
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume55
Issue number4
DOIs
Publication statusPublished - Apr 1 1999

ASJC Scopus subject areas

  • Structural Biology

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