Abstract
Tetrathionate hydrolase (4THase) from the iron- and sulfur-oxidizing bacterium Acidithiobacillus ferrooxidans catalyses the disproportionate hydrolysis of tetrathionate to elemental sulfur, thiosulfate and sulfate. The gene encoding 4THase (Af-tth) was expressed as inclusion bodies in recombinant Escherichia coli. Recombinant Af-Tth was activated by refolding under acidic conditions and was then purified to homogeneity. The recombinant protein was crystallized in 20 mM glycine buffer pH 10 containing 50 mM sodium chloride and 33%(v/v) PEG 1000 using the hanging-drop vapour-diffusion method. The crystal was a hexagonal cylinder with dimensions of 0.2 × 0.05 × 0.05 mm. X-ray crystallographic analysis showed that the crystal diffracted to 2.15 Å resolution and belongs to space group P31 or P32, with unit-cell parameters a = b = 92.1, c = 232.6 Å.
| Original language | English |
|---|---|
| Pages (from-to) | 692-694 |
| Number of pages | 3 |
| Journal | Acta Crystallographica Section F: Structural Biology and Crystallization Communications |
| Volume | 69 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2013 |
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Keywords
- Acidithiobacillus ferrooxidans
- tetrathionate hydrolase
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Structural Biology
- Genetics
- Condensed Matter Physics
Cite this
Crystallization and preliminary X-ray diffraction analysis of tetrathionate hydrolase from Acidithiobacillus ferrooxidans. / Kanao, Tadayoshi; Kosaka, Megumi; Yoshida, Kyoya; Nakayama, Hisayuki; Tamada, Taro; Kuroki, Ryota; Yamada, Hidenori; Takada, Jun; Kamimura, Kazuo.
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 69, No. 6, 2013, p. 692-694.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Crystallization and preliminary X-ray diffraction analysis of tetrathionate hydrolase from Acidithiobacillus ferrooxidans
AU - Kanao, Tadayoshi
AU - Kosaka, Megumi
AU - Yoshida, Kyoya
AU - Nakayama, Hisayuki
AU - Tamada, Taro
AU - Kuroki, Ryota
AU - Yamada, Hidenori
AU - Takada, Jun
AU - Kamimura, Kazuo
PY - 2013
Y1 - 2013
N2 - Tetrathionate hydrolase (4THase) from the iron- and sulfur-oxidizing bacterium Acidithiobacillus ferrooxidans catalyses the disproportionate hydrolysis of tetrathionate to elemental sulfur, thiosulfate and sulfate. The gene encoding 4THase (Af-tth) was expressed as inclusion bodies in recombinant Escherichia coli. Recombinant Af-Tth was activated by refolding under acidic conditions and was then purified to homogeneity. The recombinant protein was crystallized in 20 mM glycine buffer pH 10 containing 50 mM sodium chloride and 33%(v/v) PEG 1000 using the hanging-drop vapour-diffusion method. The crystal was a hexagonal cylinder with dimensions of 0.2 × 0.05 × 0.05 mm. X-ray crystallographic analysis showed that the crystal diffracted to 2.15 Å resolution and belongs to space group P31 or P32, with unit-cell parameters a = b = 92.1, c = 232.6 Å.
AB - Tetrathionate hydrolase (4THase) from the iron- and sulfur-oxidizing bacterium Acidithiobacillus ferrooxidans catalyses the disproportionate hydrolysis of tetrathionate to elemental sulfur, thiosulfate and sulfate. The gene encoding 4THase (Af-tth) was expressed as inclusion bodies in recombinant Escherichia coli. Recombinant Af-Tth was activated by refolding under acidic conditions and was then purified to homogeneity. The recombinant protein was crystallized in 20 mM glycine buffer pH 10 containing 50 mM sodium chloride and 33%(v/v) PEG 1000 using the hanging-drop vapour-diffusion method. The crystal was a hexagonal cylinder with dimensions of 0.2 × 0.05 × 0.05 mm. X-ray crystallographic analysis showed that the crystal diffracted to 2.15 Å resolution and belongs to space group P31 or P32, with unit-cell parameters a = b = 92.1, c = 232.6 Å.
KW - Acidithiobacillus ferrooxidans
KW - tetrathionate hydrolase
UR - http://www.scopus.com/inward/record.url?scp=84878612363&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84878612363&partnerID=8YFLogxK
U2 - 10.1107/S1744309113013419
DO - 10.1107/S1744309113013419
M3 - Article
C2 - 23722856
AN - SCOPUS:84878612363
VL - 69
SP - 692
EP - 694
JO - Acta Crystallographica Section F:Structural Biology Communications
JF - Acta Crystallographica Section F:Structural Biology Communications
SN - 1744-3091
IS - 6
ER -