Crystallization and preliminary cristallographic study of tropinone reductase II from Datura stramonium

Atsuko Yamashita, Keiji Nakajima, Hiroaki Kato, Takashi Hashimoto, Yasuyuki Yamada, Jun'ichi Oda

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Tropinone reductase II is an NADPH-dependent oxidoreductase involved in a plant alkaloid metabolism. The enzyme from Datura stramonium has been crystallized using 2-methyl-2,4-pentanediol as a precipitant and a macro-seeding technique. The crystal belongs to space group P42212, with cell dimensions a = b = 62.8 Å and c = 128.4 Å. A data set to 2.6 Å resolution has been collected at a cryogenic temperature.

Original languageEnglish
Pages (from-to)1405-1407
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume54
Issue number6 PART II
DOIs
Publication statusPublished - 1998
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology

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