Formate dehydrogenase (EC 18.104.22.168) was purified about 38-fold with an overall yield of 76% from a methanol-utilizing yeast, Candida methanolica (ATCC26175), in 4 steps and, by adding polyethylene glycol, the enzyme was crystallised for the first time. The final preparation appeared to be homogeneous by the criteria of polyacrylamide electrophoresis and analytical centrifugation. Compared with the yeast formate dehydrogenases so far reported, the purified enzyme exhibited higher specific activity (7.52 U/mg).
|Number of pages||4|
|Journal||FEMS Microbiology Letters|
|Publication status||Published - Dec 1 1987|
- Candida methanolica
- Formate dehydrogenase
ASJC Scopus subject areas
- Molecular Biology