Crystalline formate dehydrogenase from Candida methanolica

Yoshikazu Izumi, Hiroshi Kanzaki, Shigeru Morita, Hideaki Yamada

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Formate dehydrogenase (EC 1.2.1.2) was purified about 38-fold with an overall yield of 76% from a methanol-utilizing yeast, Candida methanolica (ATCC26175), in 4 steps and, by adding polyethylene glycol, the enzyme was crystallised for the first time. The final preparation appeared to be homogeneous by the criteria of polyacrylamide electrophoresis and analytical centrifugation. Compared with the yeast formate dehydrogenases so far reported, the purified enzyme exhibited higher specific activity (7.52 U/mg).

Original languageEnglish
Pages (from-to)139-142
Number of pages4
JournalFEMS Microbiology Letters
Volume48
Issue number1-2
DOIs
Publication statusPublished - Dec 1 1987
Externally publishedYes

Keywords

  • Candida methanolica
  • Formate dehydrogenase

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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