Crystal structure of the flexible tandem repeat domain of bacterial cellulose synthesis subunit

Shingo Nojima, Ayumi Fujishima, Koji Kato, Kayoko Ohuchi, Nobutaka Shimizu, Kento Yonezawa, Kenji Tajima, Min Yao

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Bacterial cellulose (BC) is synthesized and exported through the cell membrane via a large protein complex (terminal complex) that consists of three or four subunits. BcsC is a little-studied subunit considered to export BC to the extracellular matrix. It is predicted to have two domains: a tetratrico peptide repeat (TPR) domain and a β-barrelled outer membrane domain. Here we report the crystal structure of the N-terminal part of BcsC-TPR domain (Asp24-Arg272) derived from Enterobacter CJF-002. Unlike most TPR-containing proteins which have continuous TPR motifs, this structure has an extra α-helix between two clusters of TPR motifs. Five independent molecules in the crystal had three different conformations that varied at the hinge of the inserted α-helix. Such structural feature indicates that the inserted α-helix confers flexibility to the chain and changes the direction of the TPR super-helix, which was also suggested by structural analysis of BcsC-TPR (Asp24-Leu664) in solution by size exclusion chromatography-small-angle X-ray scattering. The flexibility at the α-helical hinge may play important role for exporting glucan chains.

Original languageEnglish
Article number13018
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - Dec 1 2017
Externally publishedYes

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Tandem Repeat Sequences
Cellulose
Crystal structure
Peptides
Hinges
Glucans
Size exclusion chromatography
Cell membranes
X ray scattering
Structural analysis
Conformations
Proteins
Membranes
Crystals
Molecules

ASJC Scopus subject areas

  • General

Cite this

Crystal structure of the flexible tandem repeat domain of bacterial cellulose synthesis subunit. / Nojima, Shingo; Fujishima, Ayumi; Kato, Koji; Ohuchi, Kayoko; Shimizu, Nobutaka; Yonezawa, Kento; Tajima, Kenji; Yao, Min.

In: Scientific Reports, Vol. 7, No. 1, 13018, 01.12.2017.

Research output: Contribution to journalArticle

Nojima, S, Fujishima, A, Kato, K, Ohuchi, K, Shimizu, N, Yonezawa, K, Tajima, K & Yao, M 2017, 'Crystal structure of the flexible tandem repeat domain of bacterial cellulose synthesis subunit', Scientific Reports, vol. 7, no. 1, 13018. https://doi.org/10.1038/s41598-017-12530-0
Nojima, Shingo ; Fujishima, Ayumi ; Kato, Koji ; Ohuchi, Kayoko ; Shimizu, Nobutaka ; Yonezawa, Kento ; Tajima, Kenji ; Yao, Min. / Crystal structure of the flexible tandem repeat domain of bacterial cellulose synthesis subunit. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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