Crystal structure of the C-terminal half of tropomodulin and structural basis of actin filament pointed-end capping

Inna Krieger, Alla Kostyukova, Atsuko Yamashita, Yasushi Nitanai, Yuichiro Maéda

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Tropomodulin is the unique pointed-end capping protein of the actin-tropomyosin filament. By blocking elongation and depolymerization, tropomodulin regulates the architecture and the dynamics of the filament. Here we report the crystal structure at 1.45-Å resolution of the C-terminal half of tropomodulin (C20), the actin-binding moiety of tropomodulin. C20 is a leucine-rich repeat domain, and this is the first actin-associated protein with a leucine-rich repeat. Binding assays suggested that C20 also interacts with the N-terminal fragment, M1-M2-M3, of nebulin. Based on the crystal structure, we propose a model for C20 docking to the actin subunit at the pointed end. Although speculative, the model is consistent with the idea that a tropomodulin molecule competes with an actin subunit for a pointed end. The model also suggests that interactions with tropomyosin, actin, and nebulin are all possible sources of influences on the dynamic properties of pointed-end capping by tropomodulin.

Original languageEnglish
Pages (from-to)2716-2725
Number of pages10
JournalBiophysical Journal
Volume83
Issue number5
Publication statusPublished - Nov 1 2002
Externally publishedYes

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Tropomodulin
Actin Cytoskeleton
Actins
Tropomyosin
Leucine
Actin Capping Proteins

ASJC Scopus subject areas

  • Biophysics

Cite this

Crystal structure of the C-terminal half of tropomodulin and structural basis of actin filament pointed-end capping. / Krieger, Inna; Kostyukova, Alla; Yamashita, Atsuko; Nitanai, Yasushi; Maéda, Yuichiro.

In: Biophysical Journal, Vol. 83, No. 5, 01.11.2002, p. 2716-2725.

Research output: Contribution to journalArticle

Krieger, Inna ; Kostyukova, Alla ; Yamashita, Atsuko ; Nitanai, Yasushi ; Maéda, Yuichiro. / Crystal structure of the C-terminal half of tropomodulin and structural basis of actin filament pointed-end capping. In: Biophysical Journal. 2002 ; Vol. 83, No. 5. pp. 2716-2725.
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