Crystal Structure of Talaromyces cellulolyticus (Formerly Known as Acremonium cellulolyticus) GH Family 11 Xylanase

Misumi Kataoka, Fusamichi Akita, Yuka Maeno, Benchaporn Inoue, Hiroyuki Inoue, Kazuhiko Ishikawa

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Talaromyces cellulolyticus (formerly known as Acremonium cellulolyticus) is one of the mesophilic fungi that can produce high levels of cellulose-related enzymes and are expected to be used for the degradation of polysaccharide biomass. In silico analysis of the genome sequence of T. cellulolyticus detected seven open reading frames (ORFs) showing homology to xylanases from glycoside hydrolase (GH) family 11. The gene encoding the GH11 xylanase C (TcXylC) with the highest activity was used for overproduction and purification of the recombinant enzyme, permitting solving of the crystal structure to a resolution of 1.98 Å. In the asymmetric unit, two kinds of the crystal structures of the xylanase were identified. The main structure of the protein showed a β-jelly roll structure. We hypothesize that one of the two structures represents the open form and the other shows the close form. The changing of the flexible region between the two structures is presumed to induce and accelerate the enzyme reaction. The specificity of xylanase toward the branched xylan is discussed in the context of this structural data and by comparison with the other published structures of xylanases.

Original languageEnglish
Pages (from-to)1599-1612
Number of pages14
JournalApplied Biochemistry and Biotechnology
Volume174
Issue number4
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Talaromyces
Acremonium
Glycoside Hydrolases
Enzymes
Crystal structure
Endo-1,4-beta Xylanases
Xylans
Gene encoding
Polysaccharides
Fungi
Cellulose
Computer Simulation
Biomass
Open Reading Frames
Purification
Sequence Analysis
Genes
Genome
Proteins
Degradation

Keywords

  • Biomass
  • Hemicellulose
  • Talaromyces cellulolyticus (formerly known as Acremonium cellulolyticus)
  • X-ray crystal structure
  • Xylanase
  • β-Jelly roll

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Medicine(all)

Cite this

Crystal Structure of Talaromyces cellulolyticus (Formerly Known as Acremonium cellulolyticus) GH Family 11 Xylanase. / Kataoka, Misumi; Akita, Fusamichi; Maeno, Yuka; Inoue, Benchaporn; Inoue, Hiroyuki; Ishikawa, Kazuhiko.

In: Applied Biochemistry and Biotechnology, Vol. 174, No. 4, 2014, p. 1599-1612.

Research output: Contribution to journalArticle

Kataoka, Misumi ; Akita, Fusamichi ; Maeno, Yuka ; Inoue, Benchaporn ; Inoue, Hiroyuki ; Ishikawa, Kazuhiko. / Crystal Structure of Talaromyces cellulolyticus (Formerly Known as Acremonium cellulolyticus) GH Family 11 Xylanase. In: Applied Biochemistry and Biotechnology. 2014 ; Vol. 174, No. 4. pp. 1599-1612.
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