Crystal structure of Arp2/3 complex

R. C. Robinson, K. Turbedsky, D. A. Kaiser, J. B. Marchand, H. N. Higgs, S. Choe, T. D. Pollard

Research output: Contribution to journalArticle

370 Citations (Scopus)

Abstract

We determined a crystal structure of bovine Arp2/3 complex, an assembly of seven proteins that initiates actin polymerization in eukaryotic cells, at 2.0 angstrom resolution. Actin-related protein 2 (Arp2) and Arp3 are folded like actin, with distinctive surface features. Subunits ARPC2 p34 and ARPC4 p20 in the core of the complex associate through long carboxyl-.terminal α helices and have similarly folded amino-terminal α/β domains. ARPC1 p40 is a seven-blade β propeller with an insertion that may associate with the side of an actin filament. ARPC3 p21 and ARPC5 p16 are globular α-helical subunits. We predict that WASp/Scar proteins activate Arp2/3 complex by bringing Arp2 into proximity with Arp3 for nucleation of a branch on the side of a preexisting actin filament.

Original languageEnglish
Pages (from-to)1679-1684
Number of pages6
JournalScience
Volume294
Issue number5547
DOIs
Publication statusPublished - Nov 23 2001
Externally publishedYes

Fingerprint

Actin-Related Protein 2
Actin-Related Protein 2-3 Complex
Actin Cytoskeleton
Actins
Eukaryotic Cells
Polymerization
Cicatrix
Proteins

ASJC Scopus subject areas

  • General

Cite this

Robinson, R. C., Turbedsky, K., Kaiser, D. A., Marchand, J. B., Higgs, H. N., Choe, S., & Pollard, T. D. (2001). Crystal structure of Arp2/3 complex. Science, 294(5547), 1679-1684. https://doi.org/10.1126/science.1066333

Crystal structure of Arp2/3 complex. / Robinson, R. C.; Turbedsky, K.; Kaiser, D. A.; Marchand, J. B.; Higgs, H. N.; Choe, S.; Pollard, T. D.

In: Science, Vol. 294, No. 5547, 23.11.2001, p. 1679-1684.

Research output: Contribution to journalArticle

Robinson, RC, Turbedsky, K, Kaiser, DA, Marchand, JB, Higgs, HN, Choe, S & Pollard, TD 2001, 'Crystal structure of Arp2/3 complex', Science, vol. 294, no. 5547, pp. 1679-1684. https://doi.org/10.1126/science.1066333
Robinson RC, Turbedsky K, Kaiser DA, Marchand JB, Higgs HN, Choe S et al. Crystal structure of Arp2/3 complex. Science. 2001 Nov 23;294(5547):1679-1684. https://doi.org/10.1126/science.1066333
Robinson, R. C. ; Turbedsky, K. ; Kaiser, D. A. ; Marchand, J. B. ; Higgs, H. N. ; Choe, S. ; Pollard, T. D. / Crystal structure of Arp2/3 complex. In: Science. 2001 ; Vol. 294, No. 5547. pp. 1679-1684.
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