Crystal structure of a crustacean hyperglycemic hormone (CHH) precursor suggests structural variety in the C-terminal regions of CHH superfamily members

Naoaki Tsutsui, Tatsuya Sakamoto, Fumio Arisaka, Masaru Tanokura, Hiromichi Nagasawa, Koji Nagata

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The crustacean hyperglycemic hormone (CHH) is one of the major hormones in crustaceans, and peptides belonging to the CHH superfamily have been found in diverse ecdysozoans. Although the basic function of CHH is to control energy metabolism, it also plays various roles in crustacean species, such as in molting and vitellogenesis. Here, we present the crystal structure of Pej-SGP-I-Gly, a partially active precursor of CHH from the kuruma prawn Marsupenaeus japonicus, which has an additional Gly residue in place of the C-terminal amide group of the mature Pej-SGP-I. The 1.6-angstrom crystal structure showed not only the common CHH superfamily scaffold comprising three α-helices, three disulfide bridges, and a hydrophobic core but also revealed that the C-terminal part has a variant backbone fold that is specific to Pej-SGP-I-Gly. The α-helix 4 of Pej-SGP-I-Gly was much longer than that of molt-inhibiting hormone (Pej-MIH) from the same species, and as a result, the following C-terminal helix, corresponding to α-helix 5 in MIH, was not formed. Unlike monomeric Pej-MIH, Pej-SGP-I-Gly forms a homodimer in the crystal structure via its unique α-helix 4. The unexpected dissimilar folds between Pej-SGP-I-Gly and Pej-MIH appear to be the result of their distinct C-terminal amino acid sequences. Variations in amino acid sequences and lengths and the resulting variety of backbone folds allow the C-terminal and sterically adjoining regions to confer different hormonal activities in diverse CHH superfamily members. Database: Structural data are available in the PDB under the accession number 5B5I.

Original languageEnglish
Pages (from-to)4325-4339
Number of pages15
JournalFEBS Journal
Volume283
Issue number23
DOIs
Publication statusPublished - Dec 1 2016

Fingerprint

Crystal structure
Amino Acid Sequence
Vitellogenesis
Amino Acids
Molting
Scaffolds
Amides
Disulfides
Energy Metabolism
crustacean hyperglycemic hormone precursor
crustacean hyperglycemic hormone
Databases
Hormones
Peptides

Keywords

  • crustacean hyperglycemic hormone family
  • crystal structure
  • invertebrate
  • Marsupenaeus japonicus
  • peptide hormone

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Crystal structure of a crustacean hyperglycemic hormone (CHH) precursor suggests structural variety in the C-terminal regions of CHH superfamily members. / Tsutsui, Naoaki; Sakamoto, Tatsuya; Arisaka, Fumio; Tanokura, Masaru; Nagasawa, Hiromichi; Nagata, Koji.

In: FEBS Journal, Vol. 283, No. 23, 01.12.2016, p. 4325-4339.

Research output: Contribution to journalArticle

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