Cryptochrome is present in the compound eyes and a subset of Drosophila's clock neurons

Taishi Yoshii, Takeshi Todo, Corinna Wülbeck, Ralf Stanewsky, Charlotte Helfrich-Förster

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

Cryptochrome (CRY) is intimately associated with the circadian clock of many organisms. In the fruit fly Drosophila melanogaster, CRY seems to be involved in photoreception as well as in the core clockwork. In spite of the critical role of CRY for the clock of Drosophila, it was not quite clear whether CRY is expressed in every clock cell. With the help of a new antibody and a mutant that lacks CRY, we show here that CRY is expressed in specific subsets of Drosophila's pacemaker neurons and in the photoreceptor cells of the compound eyes. In the pacemaker neurons, CRY levels and kinetics under light-dark cycles are quite different from each other. High-amplitude oscillations are observed in only three groups of clock neurons, suggesting that these three groups are strongly receptive to light. The different CRY kinetics may account for phase differences in oscillations of the clock proteins observed in these three groups in earlier studies. The molecular clock of the neurons that contain lower CRY levels or are completely CRY negative can still be synchronized by light, probably via intercellular communication with the CRY-positive neurons as well as via external photoreceptors.

Original languageEnglish
Pages (from-to)952-966
Number of pages15
JournalJournal of Comparative Neurology
Volume508
Issue number6
DOIs
Publication statusPublished - Jun 20 2008
Externally publishedYes

Fingerprint

Cryptochromes
Drosophila
Neurons
Light
Photoreceptor Cells
Circadian Clocks
Photoperiod
Drosophila melanogaster
Diptera
Fruit

Keywords

  • Circadian clock
  • Cryptochrome
  • Immunohistochemistry
  • Photoreception
  • Timeless

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cryptochrome is present in the compound eyes and a subset of Drosophila's clock neurons. / Yoshii, Taishi; Todo, Takeshi; Wülbeck, Corinna; Stanewsky, Ralf; Helfrich-Förster, Charlotte.

In: Journal of Comparative Neurology, Vol. 508, No. 6, 20.06.2008, p. 952-966.

Research output: Contribution to journalArticle

Yoshii, Taishi ; Todo, Takeshi ; Wülbeck, Corinna ; Stanewsky, Ralf ; Helfrich-Förster, Charlotte. / Cryptochrome is present in the compound eyes and a subset of Drosophila's clock neurons. In: Journal of Comparative Neurology. 2008 ; Vol. 508, No. 6. pp. 952-966.
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