cryptochrome genes mediate photoperiodic responses in the cricket Modicogryllus siamensis

Haruki Ueda, Saori Tamaki, Taiki Miki, Outa Uryu, Yuichi Kamae, Motoki Nose, Tsugumichi Shinohara, Kenji Tomioka

Research output: Contribution to journalArticle

Abstract

Diapause is a strategy used by many insects living in temperate zones to survive the winter. The most reliable environmental cue to induce diapause is photoperiod, for which day or night length is measured by a mechanism called the photoperiodic clock. Despite several studies in insects, the photoperiodic mechanisms remain unclear. In the present study, we show that cryptochromes (crys) mediate photoperiodic responses in the cricket Modicogryllus siamensis. Nymphal development of M. siamensis is highly dependent on photoperiod: under long-day conditions, most nymphs become adults within 60 days after hatching by undergoing seven moults, whereas, under short-day conditions, they take much longer (approximately 180 days), with an increased number of moults. Similar to most insects, the cricket has two cry genes: the Drosophila type cry (Ms'cry1) and the mammalian type cry (Ms'cry2). Ms'cry1 shows some constitutive expression, whereas Ms'cry2 is rhythmically expressed under both long-day and short-day conditions. Parental RNA interference against either or both of the two cry genes partially prevents the long-day responses, whereas inhibition of Ms'cry1 or Ms'cry1/Ms'cry2 enhances the short-day response with a further delay of adult emergence or its complete prevention, respectively. Nymphal RNA interference of Ms'crys at the fourth-instar stage delays adult emergence and increases the number of moults under long-day conditions. We hypothesize that Ms'cry genes are involved not only in the photoperiodic mechanism, but also in the regulatory mechanism of nymphal development downstream of the photoperiodic clock in the cricket M. siamensis.

Original languageEnglish
Pages (from-to)285-294
Number of pages10
JournalPhysiological Entomology
Volume43
Issue number4
DOIs
Publication statusPublished - Dec 1 2018

Fingerprint

Cryptochromes
Gryllidae
cricket
molt
molting
Insects
diapause
Photoperiod
insect
RNA Interference
photoperiod
RNA interference
insects
eclosion
RNA
gene
Genes
Nymph
environmental cue
genes

Keywords

  • Cricket
  • cryptochrome
  • nymphal development
  • photoperiodism

ASJC Scopus subject areas

  • Physiology
  • Ecology, Evolution, Behavior and Systematics
  • Insect Science

Cite this

cryptochrome genes mediate photoperiodic responses in the cricket Modicogryllus siamensis. / Ueda, Haruki; Tamaki, Saori; Miki, Taiki; Uryu, Outa; Kamae, Yuichi; Nose, Motoki; Shinohara, Tsugumichi; Tomioka, Kenji.

In: Physiological Entomology, Vol. 43, No. 4, 01.12.2018, p. 285-294.

Research output: Contribution to journalArticle

Ueda, H, Tamaki, S, Miki, T, Uryu, O, Kamae, Y, Nose, M, Shinohara, T & Tomioka, K 2018, 'cryptochrome genes mediate photoperiodic responses in the cricket Modicogryllus siamensis', Physiological Entomology, vol. 43, no. 4, pp. 285-294. https://doi.org/10.1111/phen.12258
Ueda, Haruki ; Tamaki, Saori ; Miki, Taiki ; Uryu, Outa ; Kamae, Yuichi ; Nose, Motoki ; Shinohara, Tsugumichi ; Tomioka, Kenji. / cryptochrome genes mediate photoperiodic responses in the cricket Modicogryllus siamensis. In: Physiological Entomology. 2018 ; Vol. 43, No. 4. pp. 285-294.
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