Cryptic CTL epitope on a murine sarcoma Meth A generated by exon extension as a novel mechanism

Akiko Uenaka, Yoshiki Hirano, Hidenori Hata, Sanda Win, Toshiki Aji, Motoyuki Tanaka, Toshiro Ono, Jonathan C A Skipper, Kenji Shimizu, Eiichi Nakayama

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Using the recently developed ELISPOT cloning methodology, we obtained cDNA clone S35 coding for the Ag epitope recognized by a murine sarcoma Meth A-specific CTL clone AT-1. Analysis of truncated S35 constructs and overlapping peptides revealed that the peptide epitope was LGAEAIFRL. AT-1 CTL lysed peptide-pulsed CMS8 cells at a nanomolar concentration, and the peptide strongly stimulated IFN-γ production in AT-1 CTL. Sequence homology indicated that the S35 was derived from a mouse homologue of human retinoic acid-regulated nuclear matrix-associated protein (ramp). The ramp gene consisted of 15 exons. The majority of the ramp mRNA was the transcript normally spliced between exons 14 and 15, but a minor population of mRNA with an extended exon 14 was also present in Meth A cells. The epitope was derived from the newly created open reading frame, which resulted from extension of exon 14 after splicing of the adjacent intronic sequence.

Original languageEnglish
Pages (from-to)4862-4868
Number of pages7
JournalJournal of Immunology
Volume170
Issue number9
Publication statusPublished - May 1 2003

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Nuclear Matrix-Associated Proteins
Sarcoma
Epitopes
Exons
Peptides
Clone Cells
Enzyme-Linked Immunospot Assay
Messenger RNA
Sequence Homology
Tretinoin
Open Reading Frames
Organism Cloning
Complementary DNA
Population
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Uenaka, A., Hirano, Y., Hata, H., Win, S., Aji, T., Tanaka, M., ... Nakayama, E. (2003). Cryptic CTL epitope on a murine sarcoma Meth A generated by exon extension as a novel mechanism. Journal of Immunology, 170(9), 4862-4868.

Cryptic CTL epitope on a murine sarcoma Meth A generated by exon extension as a novel mechanism. / Uenaka, Akiko; Hirano, Yoshiki; Hata, Hidenori; Win, Sanda; Aji, Toshiki; Tanaka, Motoyuki; Ono, Toshiro; Skipper, Jonathan C A; Shimizu, Kenji; Nakayama, Eiichi.

In: Journal of Immunology, Vol. 170, No. 9, 01.05.2003, p. 4862-4868.

Research output: Contribution to journalArticle

Uenaka, A, Hirano, Y, Hata, H, Win, S, Aji, T, Tanaka, M, Ono, T, Skipper, JCA, Shimizu, K & Nakayama, E 2003, 'Cryptic CTL epitope on a murine sarcoma Meth A generated by exon extension as a novel mechanism', Journal of Immunology, vol. 170, no. 9, pp. 4862-4868.
Uenaka A, Hirano Y, Hata H, Win S, Aji T, Tanaka M et al. Cryptic CTL epitope on a murine sarcoma Meth A generated by exon extension as a novel mechanism. Journal of Immunology. 2003 May 1;170(9):4862-4868.
Uenaka, Akiko ; Hirano, Yoshiki ; Hata, Hidenori ; Win, Sanda ; Aji, Toshiki ; Tanaka, Motoyuki ; Ono, Toshiro ; Skipper, Jonathan C A ; Shimizu, Kenji ; Nakayama, Eiichi. / Cryptic CTL epitope on a murine sarcoma Meth A generated by exon extension as a novel mechanism. In: Journal of Immunology. 2003 ; Vol. 170, No. 9. pp. 4862-4868.
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