Cryptic CTL epitope on a murine sarcoma Meth A generated by exon extension as a novel mechanism

Akiko Uenaka, Yoshiki Hirano, Hidenori Hata, Sanda Win, Toshiki Aji, Motoyuki Tanaka, Toshiro Ono, Jonathan C.A. Skipper, Kenji Shimizu, Eiichi Nakayama

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Using the recently developed ELISPOT cloning methodology, we obtained cDNA clone S35 coding for the Ag epitope recognized by a murine sarcoma Meth A-specific CTL clone AT-1. Analysis of truncated S35 constructs and overlapping peptides revealed that the peptide epitope was LGAEAIFRL. AT-1 CTL lysed peptide-pulsed CMS8 cells at a nanomolar concentration, and the peptide strongly stimulated IFN-γ production in AT-1 CTL. Sequence homology indicated that the S35 was derived from a mouse homologue of human retinoic acid-regulated nuclear matrix-associated protein (ramp). The ramp gene consisted of 15 exons. The majority of the ramp mRNA was the transcript normally spliced between exons 14 and 15, but a minor population of mRNA with an extended exon 14 was also present in Meth A cells. The epitope was derived from the newly created open reading frame, which resulted from extension of exon 14 after splicing of the adjacent intronic sequence.

Original languageEnglish
Pages (from-to)4862-4868
Number of pages7
JournalJournal of Immunology
Volume170
Issue number9
DOIs
Publication statusPublished - May 1 2003

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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