Cryopreservation of ovaries from neonatal marmoset monkeys

Hideyuki H. Motohashi, Hidetoshi Ishibashi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The ovary of neonatal nonhuman primates contains the highest number of immature oocytes, but its cryopreservation has not yet been sufficiently investigated in all life stages. In the current study, we investigated cryodamage after vitrification/warming of neonatal ovaries from a nonhuman primate, the common marmoset (Callithrix jacchus). A Cryotop was used for cryopreservation of whole ovaries. The morphology of the vitrified/warmed ovaries was found to be equivalent to that of fresh ovaries. No significant difference in the number of oocytes retaining normal morphology per unit area in histological sections was found between the two groups. In an analysis of dispersed cells from the ovaries, however, the cell viability of the vitrified/warmed group tended to be decreased. The results of a comet assay showed no significant differences in DNA damage. These results show that cryopreservation of neonatal marmoset ovaries using vitrification may be useful as a storage system for whole ovaries.

Original languageEnglish
Pages (from-to)189-196
Number of pages8
JournalExperimental Animals
Volume65
Issue number3
Publication statusPublished - 2016

Fingerprint

Callithrix
Vitrification
Callitrichidae
Cryopreservation
cryopreservation
Haplorhini
monkeys
Ovary
Callithrix jacchus
vitrification
Primates
oocytes
Assays
Cells
DNA damage
cell viability
DNA
immatures
Oocytes
assays

Keywords

  • Comet assay
  • Common marmoset
  • Neonate
  • Ovarian tissue cryopreservation
  • Vitrification

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Motohashi, H. H., & Ishibashi, H. (2016). Cryopreservation of ovaries from neonatal marmoset monkeys. Experimental Animals, 65(3), 189-196.

Cryopreservation of ovaries from neonatal marmoset monkeys. / Motohashi, Hideyuki H.; Ishibashi, Hidetoshi.

In: Experimental Animals, Vol. 65, No. 3, 2016, p. 189-196.

Research output: Contribution to journalArticle

Motohashi, HH & Ishibashi, H 2016, 'Cryopreservation of ovaries from neonatal marmoset monkeys', Experimental Animals, vol. 65, no. 3, pp. 189-196.
Motohashi, Hideyuki H. ; Ishibashi, Hidetoshi. / Cryopreservation of ovaries from neonatal marmoset monkeys. In: Experimental Animals. 2016 ; Vol. 65, No. 3. pp. 189-196.
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