Cryo-electron tomography: Moving towards revealing the viral life cycle of Rice dwarf virus

Naoyuki Miyazaki, Fusamichi Akita, Atsushi Nakagawa, Kazuyoshi Murata, Toshihiro Omura, Kenji Iwasaki

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

It is well known that viruses utilize the host cellular systems for their infection and replication processes. However, the molecular mechanisms underlying these processes are poorly understood for most viruses. To understand these molecular mechanisms, it is essential to observe the viral and virus-related structures and analyse their molecular interactions within a cellular context. Cryo-electron microscopy and tomography offer the potential to observe macromolecular structures and to analyse their molecular interactions within the cell. Here, using cryo-electron microscopy and tomography, the structures of Rice dwarf virus are reported within fully hydrated insect vector cells grown on electron microscopy grids towards revealing the viral infection and replication mechanisms.

Original languageEnglish
Pages (from-to)826-828
Number of pages3
JournalJournal of Synchrotron Radiation
Volume20
Issue number6
DOIs
Publication statusPublished - Nov 2013

Fingerprint

viruses
rice
Viruses
Tomography
Life cycle
tomography
Electron microscopy
electron microscopy
cycles
Molecular interactions
Electrons
infectious diseases
molecular interactions
electrons
insects
grids
Cells
cells

Keywords

  • cryo-electron microscopy
  • cryo-electron tomography
  • Phytoreovirus
  • Rice dwarf virus
  • virus structure

ASJC Scopus subject areas

  • Instrumentation
  • Nuclear and High Energy Physics
  • Radiation

Cite this

Cryo-electron tomography : Moving towards revealing the viral life cycle of Rice dwarf virus. / Miyazaki, Naoyuki; Akita, Fusamichi; Nakagawa, Atsushi; Murata, Kazuyoshi; Omura, Toshihiro; Iwasaki, Kenji.

In: Journal of Synchrotron Radiation, Vol. 20, No. 6, 11.2013, p. 826-828.

Research output: Contribution to journalArticle

Miyazaki, Naoyuki ; Akita, Fusamichi ; Nakagawa, Atsushi ; Murata, Kazuyoshi ; Omura, Toshihiro ; Iwasaki, Kenji. / Cryo-electron tomography : Moving towards revealing the viral life cycle of Rice dwarf virus. In: Journal of Synchrotron Radiation. 2013 ; Vol. 20, No. 6. pp. 826-828.
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