Cripto-1 induces apoptosis in HC-11 mouse mammary epithelial cells

M. L. De Santis, I. Martinez-Lacaci, C. Bianco, Masaharu Seno, B. Wallace-Jones, N. Kim, A. Ebert, C. Wechselberger, D. S. Salomon

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Cripto-1 (CR-1) is an epidermal growth factor (EGF)-related protein. CR-1 can inhibit β-casein and whey acidic protein expression in mouse mammary epithelial cells. The present study demonstrates that CR-1 can induce apoptosis in HC-11 mouse mammary epithelial cells, as measured by bis-benzimide stained nuclei, TUNEL assay and cell death ELISA. Apoptosis could be observed after 2 days of exposure of confluent HC-11 cells to CR-1 in the absence of the survival factors EGF and insulin, with maximum apoptosis occurring at 3 days. A reduction in poly(ADP-ribose) polymerase (PARP) expression and an increase in β-catenin cleavage was found after 18 h of exposure to CR-1 suggesting that apoptosis was preceded by the induction of a caspase activity since the caspase inhibitor ZFAD.FMK could block the CR-1-induced reduction in PARP expression and CR-1-induced apoptosis. CR-1 was found to increase the expression of caspase-3-like protease. Although, the levels of p27(kip1) and p21Bax did not change after exposure to CR-1 for 18 h, the levels of Bcl-x(L) became undetectable. These studies suggest that CR-1 promotes apoptosis by mediating the induction of caspase-3-like protease and downregulating the expression of Bcl-xL.

Original languageEnglish
Pages (from-to)189-196
Number of pages8
JournalCell Death and Differentiation
Volume7
Issue number2
Publication statusPublished - Feb 2000

Fingerprint

Breast
Epithelial Cells
Apoptosis
Poly(ADP-ribose) Polymerases
Epidermal Growth Factor
Caspase 3
Peptide Hydrolases
Catenins
Caspase Inhibitors
In Situ Nick-End Labeling
Caspases
Caseins
Cell Death
Down-Regulation
Enzyme-Linked Immunosorbent Assay
Insulin
Proteins

Keywords

  • Apoptosis
  • Caspase activation
  • Cripto-1
  • Mammary epithelial cells

ASJC Scopus subject areas

  • Cell Biology

Cite this

De Santis, M. L., Martinez-Lacaci, I., Bianco, C., Seno, M., Wallace-Jones, B., Kim, N., ... Salomon, D. S. (2000). Cripto-1 induces apoptosis in HC-11 mouse mammary epithelial cells. Cell Death and Differentiation, 7(2), 189-196.

Cripto-1 induces apoptosis in HC-11 mouse mammary epithelial cells. / De Santis, M. L.; Martinez-Lacaci, I.; Bianco, C.; Seno, Masaharu; Wallace-Jones, B.; Kim, N.; Ebert, A.; Wechselberger, C.; Salomon, D. S.

In: Cell Death and Differentiation, Vol. 7, No. 2, 02.2000, p. 189-196.

Research output: Contribution to journalArticle

De Santis, ML, Martinez-Lacaci, I, Bianco, C, Seno, M, Wallace-Jones, B, Kim, N, Ebert, A, Wechselberger, C & Salomon, DS 2000, 'Cripto-1 induces apoptosis in HC-11 mouse mammary epithelial cells', Cell Death and Differentiation, vol. 7, no. 2, pp. 189-196.
De Santis ML, Martinez-Lacaci I, Bianco C, Seno M, Wallace-Jones B, Kim N et al. Cripto-1 induces apoptosis in HC-11 mouse mammary epithelial cells. Cell Death and Differentiation. 2000 Feb;7(2):189-196.
De Santis, M. L. ; Martinez-Lacaci, I. ; Bianco, C. ; Seno, Masaharu ; Wallace-Jones, B. ; Kim, N. ; Ebert, A. ; Wechselberger, C. ; Salomon, D. S. / Cripto-1 induces apoptosis in HC-11 mouse mammary epithelial cells. In: Cell Death and Differentiation. 2000 ; Vol. 7, No. 2. pp. 189-196.
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