Cripto-1 indirectly stimulates the tyrosine phosphorylation of erb B-4 through a novel receptor

Caterina Bianco, Subha Kannan, Marta De Santis, Masaharu Seno, Careen K. Tang, Isabel Martinez-Lacaci, Nancy Kim, Brenda Wallace-Jones, Marc E. Lippman, Andreas D. Ebert, Christian Wechselberger, David S. Salomon

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Cripto-1 (CR-1) is a recently discovered protein of the epidermal growth factor family that fails to directly bind to any of the four known erb B type 1 receptor tyrosine kinases. The present study demonstrates that CR-1 indirectly induces tyrosine phosphorylation of erb B-4 but not of the epidermal growth factor-related receptors erb B-2 and erb B-3 in different mouse and human mammary epithelial cell lines. In addition, down-regulation of erb B-4 in NMuMG mouse mammary epithelial cells and in T47D human breast cancer cells, using an anti-orb B-4 blocking antibody or a hammerhead ribozyme vector targeted to orb B-4 mRNA, impairs the ability of CR-1 to fully activate mitogen-activated protein kinase. Finally, chemical cross- linking of 125I-CR-1 to mouse and human mammary epithelial cell membranes results in the labeling of two specific bands with a molecular weight of 130 and 60 kDa, suggesting that the CR-1 receptor represents a novel receptor structurally unrelated to any of the known type I receptor tyrosine kinases. In conclusion, these data demonstrate that CR-1, upon binding to an unknown receptor, can enhance the tyrosine kinase activity of erb B-4 and that a functional erb B-4 receptor is required for CR-1-induced MAPK activation.

Original languageEnglish
Pages (from-to)8624-8629
Number of pages6
JournalJournal of Biological Chemistry
Volume274
Issue number13
DOIs
Publication statusPublished - Mar 26 1999

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Phosphorylation
Tyrosine
Breast
Epithelial Cells
Receptor Protein-Tyrosine Kinases
Epidermal Growth Factor
Blocking Antibodies
Cell membranes
Mitogen-Activated Protein Kinases
Crosslinking
Protein-Tyrosine Kinases
Labeling
Down-Regulation
Molecular Weight
Chemical activation
Molecular weight
Cells
Cell Membrane
Breast Neoplasms
Cell Line

ASJC Scopus subject areas

  • Biochemistry

Cite this

Bianco, C., Kannan, S., De Santis, M., Seno, M., Tang, C. K., Martinez-Lacaci, I., ... Salomon, D. S. (1999). Cripto-1 indirectly stimulates the tyrosine phosphorylation of erb B-4 through a novel receptor. Journal of Biological Chemistry, 274(13), 8624-8629. https://doi.org/10.1074/jbc.274.13.8624

Cripto-1 indirectly stimulates the tyrosine phosphorylation of erb B-4 through a novel receptor. / Bianco, Caterina; Kannan, Subha; De Santis, Marta; Seno, Masaharu; Tang, Careen K.; Martinez-Lacaci, Isabel; Kim, Nancy; Wallace-Jones, Brenda; Lippman, Marc E.; Ebert, Andreas D.; Wechselberger, Christian; Salomon, David S.

In: Journal of Biological Chemistry, Vol. 274, No. 13, 26.03.1999, p. 8624-8629.

Research output: Contribution to journalArticle

Bianco, C, Kannan, S, De Santis, M, Seno, M, Tang, CK, Martinez-Lacaci, I, Kim, N, Wallace-Jones, B, Lippman, ME, Ebert, AD, Wechselberger, C & Salomon, DS 1999, 'Cripto-1 indirectly stimulates the tyrosine phosphorylation of erb B-4 through a novel receptor', Journal of Biological Chemistry, vol. 274, no. 13, pp. 8624-8629. https://doi.org/10.1074/jbc.274.13.8624
Bianco, Caterina ; Kannan, Subha ; De Santis, Marta ; Seno, Masaharu ; Tang, Careen K. ; Martinez-Lacaci, Isabel ; Kim, Nancy ; Wallace-Jones, Brenda ; Lippman, Marc E. ; Ebert, Andreas D. ; Wechselberger, Christian ; Salomon, David S. / Cripto-1 indirectly stimulates the tyrosine phosphorylation of erb B-4 through a novel receptor. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 13. pp. 8624-8629.
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