Counteraction by repetitive daily exposure to static magnetism against sustained blockade of N-methyl-D-aspartate receptor channels in cultured rat hippocampal neurons

Takao Hirai, Hideo Taniura, Yasuaki Goto, Keisuke Tamaki, Hirotaka Oikawa, Yuki Kambe, Masato Ogura, Yu Ohno, Takeshi Takarada, Yukio Yoneda

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In rat hippocampal neurons cultured with the antagonist for N-methyl-D-aspartate (NMDA) receptors dizocilpine (MK-801) for 8 days in vitro (DIV), a significant decrease was seen in the expression of microtubule- associated protein-2 (MAP-2) as well as mRNA for both brain-derived neurotrophic factor (BDNF) and growth-associated protein-43 (GAP-43), in addition to decreased viability. MK-801 not only decreased the expression of the NR1 subunit of NMDA receptors but also increased NR2A expression, without affecting NR2B expression. Repetitive daily exposure to static magnetic fields at 100 mT for 15 min led to a decrease in the expression of MAP-2, without significantly affecting cell viability or the expression of neuronal nuclei (NeuN) and GAP-43. However, the repetitive magnetism prevented decreases in both BDNF mRNA and MAP-2 and additionally increased the expression of NR2A subunit, without altering NR1 expression in neurons cultured in the presence of MK-801. Repetitive magnetism was also effective in preventing the decrease by MK-801 in the ability of NMDA to increase intracellular free Ca2+ ions, without affecting the decrease in the maximal response. These results suggest that repetitive magnetism may at least in part counteract the neurotoxicity of MK-801 through modulation of the expression of particular NMDA receptor subunits in cultured rat hippocampal neurons.

Original languageEnglish
Pages (from-to)491-500
Number of pages10
JournalJournal of Neuroscience Research
Volume80
Issue number4
DOIs
Publication statusPublished - May 15 2005
Externally publishedYes

Fingerprint

Dizocilpine Maleate
N-Methyl-D-Aspartate Receptors
Neurons
Microtubule-Associated Proteins
GAP-43 Protein
Brain-Derived Neurotrophic Factor
Messenger RNA
Nerve Growth Factors
N-Methylaspartate
Magnetic Fields
Cell Survival
Ions

Keywords

  • BDNF
  • Intracellular free calcium
  • MAP-2
  • MK-801
  • NMDAR
  • Static magnetic fields

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Counteraction by repetitive daily exposure to static magnetism against sustained blockade of N-methyl-D-aspartate receptor channels in cultured rat hippocampal neurons. / Hirai, Takao; Taniura, Hideo; Goto, Yasuaki; Tamaki, Keisuke; Oikawa, Hirotaka; Kambe, Yuki; Ogura, Masato; Ohno, Yu; Takarada, Takeshi; Yoneda, Yukio.

In: Journal of Neuroscience Research, Vol. 80, No. 4, 15.05.2005, p. 491-500.

Research output: Contribution to journalArticle

Hirai, Takao ; Taniura, Hideo ; Goto, Yasuaki ; Tamaki, Keisuke ; Oikawa, Hirotaka ; Kambe, Yuki ; Ogura, Masato ; Ohno, Yu ; Takarada, Takeshi ; Yoneda, Yukio. / Counteraction by repetitive daily exposure to static magnetism against sustained blockade of N-methyl-D-aspartate receptor channels in cultured rat hippocampal neurons. In: Journal of Neuroscience Research. 2005 ; Vol. 80, No. 4. pp. 491-500.
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