Cotton GhCKI disrupts normal male reproduction by delaying tapetum programmed cell death via inactivating starch synthase

Ling Min, Longfu Zhu, Lili Tu, Fenglin Deng, Daojun Yuan, Xianlong Zhang

    Research output: Contribution to journalArticlepeer-review

    47 Citations (Scopus)

    Abstract

    Anther infertility under high temperature (HT) conditions is a critical factor contributing to yield loss in cotton (Gossypium hirsutum). Using large-scale expression profile sequencing, we studied the effect of HT on cotton anther development. Our analysis revealed that altered carbohydrate metabolism or disrupted tapetal programmed cell death (PCD) underlie anther sterility. Expression of the Gossypium hirsutum casein kinase I (GhCKI) gene, which encodes a homolog of casein kinase I (CKI), was induced in an HT-sensitive cotton line after exposure to HT. As mammalian homologs of GhCKI are involved in inactivation of glycogen synthase and the regulation of apoptosis, GhCKI may be considered a target gene for improving anther fertility under HT conditions. Our studies suggest that GhCKI exhibits starch synthase kinase activity, increases glucose content in early-stage buds and activates the accumulation of abscisic acid, thereby disturbing the balance of reactive oxygen species and eventually disrupting tapetal PCD, leading to anther abortion or indehiscence. These results indicate that GhCKI may be a key regulator of tapetal PCD and anther dehiscence, with the potential to facilitate regulation of HT tolerance in crops.

    Original languageEnglish
    Pages (from-to)823-835
    Number of pages13
    JournalPlant Journal
    Volume75
    Issue number5
    DOIs
    Publication statusPublished - Sep 1 2013

    Keywords

    • Arabidopsis
    • Gossypium hirsutum
    • anther abortion
    • casein kinase I
    • high temperature
    • programmed cell death
    • starch synthase

    ASJC Scopus subject areas

    • Genetics
    • Plant Science
    • Cell Biology

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