Cotton GhCKI disrupts normal male reproduction by delaying tapetum programmed cell death via inactivating starch synthase

Ling Min, Longfu Zhu, Lili Tu, Fenglin Deng, Daojun Yuan, Xianlong Zhang

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Anther infertility under high temperature (HT) conditions is a critical factor contributing to yield loss in cotton (Gossypium hirsutum). Using large-scale expression profile sequencing, we studied the effect of HT on cotton anther development. Our analysis revealed that altered carbohydrate metabolism or disrupted tapetal programmed cell death (PCD) underlie anther sterility. Expression of the Gossypium hirsutum casein kinase I (GhCKI) gene, which encodes a homolog of casein kinase I (CKI), was induced in an HT-sensitive cotton line after exposure to HT. As mammalian homologs of GhCKI are involved in inactivation of glycogen synthase and the regulation of apoptosis, GhCKI may be considered a target gene for improving anther fertility under HT conditions. Our studies suggest that GhCKI exhibits starch synthase kinase activity, increases glucose content in early-stage buds and activates the accumulation of abscisic acid, thereby disturbing the balance of reactive oxygen species and eventually disrupting tapetal PCD, leading to anther abortion or indehiscence. These results indicate that GhCKI may be a key regulator of tapetal PCD and anther dehiscence, with the potential to facilitate regulation of HT tolerance in crops.

Original languageEnglish
Pages (from-to)823-835
Number of pages13
JournalPlant Journal
Volume75
Issue number5
DOIs
Publication statusPublished - Sep 2013
Externally publishedYes

Fingerprint

Starch Synthase
Casein Kinase I
Gossypium
starch synthase
Gossypium hirsutum
anthers
Reproduction
Cell Death
apoptosis
cotton
Temperature
temperature
Infertility
glycogen (starch) synthase
Glycogen Synthase
Abscisic Acid
abortion (plants)
dehiscence
Carbohydrate Metabolism
carbohydrate metabolism

Keywords

  • anther abortion
  • Arabidopsis
  • casein kinase I
  • Gossypium hirsutum
  • high temperature
  • programmed cell death
  • starch synthase

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics

Cite this

Cotton GhCKI disrupts normal male reproduction by delaying tapetum programmed cell death via inactivating starch synthase. / Min, Ling; Zhu, Longfu; Tu, Lili; Deng, Fenglin; Yuan, Daojun; Zhang, Xianlong.

In: Plant Journal, Vol. 75, No. 5, 09.2013, p. 823-835.

Research output: Contribution to journalArticle

Min, Ling ; Zhu, Longfu ; Tu, Lili ; Deng, Fenglin ; Yuan, Daojun ; Zhang, Xianlong. / Cotton GhCKI disrupts normal male reproduction by delaying tapetum programmed cell death via inactivating starch synthase. In: Plant Journal. 2013 ; Vol. 75, No. 5. pp. 823-835.
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