Correlation of neutrophil and monocyte derived interleukin-1 receptor antagonist and interleukin-8 with colitis severity in the rabbit

Kenta Kaneda, Fumio Saitoh, Hirom Shibusawa, Hidetaka Maegawa, Abbi R. Saniabadi, Nobuhito Kashiwagi, Akihiro Matsukawa

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Activated neutrophils and monocytes produce interleukin (IL)-8, a pro-inflammatory chemokine, but also IL-1 receptor antagonist (IL-1ra), which is an anti-inflammatory cytokine. We were interested to see the profiles of IL-8 and IL-1ra in the colonic tissue and in the peripheral blood leukocytes (PBL) during the development of immune complex induced colitis in rabbits. IL-1ra and IL-8 in PBL were measured in 26 rabbits at time 0h, 24h, and 48h after induction of colitis. The colons were removed at 48h for measuring myeloperoxidase (MPO), ulcer area, IL-1ra and IL-8. Epithelial damage, crypt abscess formation and leukocyte infiltration of the colonic tissue were major features of this colitis model. During the development of colitis, there was an increase in circulating neutrophils and monocytes (Ps=0.6327, PHigh group (n=11) showed increased IL-1ra (P=0.027) and IL-8 (P=0.0128) levels vs MPO Low group (n=15). IL-8 production by PBL showed correlation with tissue MPO (R s=0.4273, P=0.0295). The colitis in this model was associated with an increase in circulating monocytes and neutrophils, which released increased amounts of IL-8 and IL-1ra. Further, IL-8 and IL-1ra showed correlation with the severity of colitis. These observations should significantly further understandings on the role of neutrophils and monocytes in the immunopathogenesis of ulcerative colitis.

Original languageEnglish
Pages (from-to)508-514
Number of pages7
JournalCytokine
Volume56
Issue number2
DOIs
Publication statusPublished - Nov 2011

Fingerprint

Interleukin-1 Receptors
Colitis
Interleukin-8
Monocytes
Interleukins
Neutrophils
Rabbits
Leukocytes
Peroxidase
Blood
Tissue
Antigen-Antibody Complex
Ulcerative Colitis
Chemokines
Infiltration
Abscess
Ulcer
Colon
Anti-Inflammatory Agents
Cytokines

Keywords

  • Animal model of colitis
  • Cytokine
  • Inflammatory bowel disease
  • Interleukin-1 receptor antagonist
  • Interleukin-8

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Hematology
  • Biochemistry
  • Molecular Biology

Cite this

Correlation of neutrophil and monocyte derived interleukin-1 receptor antagonist and interleukin-8 with colitis severity in the rabbit. / Kaneda, Kenta; Saitoh, Fumio; Shibusawa, Hirom; Maegawa, Hidetaka; Saniabadi, Abbi R.; Kashiwagi, Nobuhito; Matsukawa, Akihiro.

In: Cytokine, Vol. 56, No. 2, 11.2011, p. 508-514.

Research output: Contribution to journalArticle

Kaneda, Kenta ; Saitoh, Fumio ; Shibusawa, Hirom ; Maegawa, Hidetaka ; Saniabadi, Abbi R. ; Kashiwagi, Nobuhito ; Matsukawa, Akihiro. / Correlation of neutrophil and monocyte derived interleukin-1 receptor antagonist and interleukin-8 with colitis severity in the rabbit. In: Cytokine. 2011 ; Vol. 56, No. 2. pp. 508-514.
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