Correlation of biological properties with glucan-binding protein B expression profile in Streptococcus mutans clinical isolates

K. Fujita, Yukiko Takashima, S. Inagaki, K. Nagayama, R. Nomura, A. C. Ardin, L. Grönroos, S. Alaluusua, T. Ooshima, Michiyo Nakano

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective: Streptococcus mutans is known to be a primary causative agent of dental caries and its surface proteins have been investigated to specify their association with its virulence. Amongst those, 4 glucan-binding proteins (Gbps) are considered to be important factors due to their glucan-binding properties, of which GbpB has been shown to participate in cell-wall construction and cell separation. Design: We examined clinical isolates of S. mutans collected from the oral cavities of Japanese and Finnish subjects, and focused on the association of their GbpB expression profiles and biological properties related to virulence. Results: Western blot analysis of GbpB expression by the isolates revealed a variety of patterns. Strains that showed single and multiple bands were used to designate S and M type strains, respectively, whilst those with no GbpB expression were classified as N type. The distribution of GbpB expression patterns was shown to be quite different between the Japanese and Finnish isolates. Furthermore, the chain length and doubling time of the N type in both populations were significantly longer than those of the other types. Conclusion: Our results suggest variations in S. mutans GbpB expression patterns, which may have relationships with the virulence of S. mutans.

Original languageEnglish
Pages (from-to)258-263
Number of pages6
JournalArchives of Oral Biology
Volume56
Issue number3
DOIs
Publication statusPublished - Mar 2011
Externally publishedYes

Fingerprint

Streptococcus mutans
Virulence
Glucans
Cell Separation
Dental Caries
Cell Wall
Mouth
Membrane Proteins
Western Blotting
glucan-binding proteins
IgA receptor
Population

Keywords

  • Clinical isolates
  • Expression
  • Glucan-binding protein B
  • Streptococcus mutans

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Cell Biology
  • Dentistry(all)

Cite this

Correlation of biological properties with glucan-binding protein B expression profile in Streptococcus mutans clinical isolates. / Fujita, K.; Takashima, Yukiko; Inagaki, S.; Nagayama, K.; Nomura, R.; Ardin, A. C.; Grönroos, L.; Alaluusua, S.; Ooshima, T.; Nakano, Michiyo.

In: Archives of Oral Biology, Vol. 56, No. 3, 03.2011, p. 258-263.

Research output: Contribution to journalArticle

Fujita, K, Takashima, Y, Inagaki, S, Nagayama, K, Nomura, R, Ardin, AC, Grönroos, L, Alaluusua, S, Ooshima, T & Nakano, M 2011, 'Correlation of biological properties with glucan-binding protein B expression profile in Streptococcus mutans clinical isolates', Archives of Oral Biology, vol. 56, no. 3, pp. 258-263. https://doi.org/10.1016/j.archoralbio.2010.09.018
Fujita, K. ; Takashima, Yukiko ; Inagaki, S. ; Nagayama, K. ; Nomura, R. ; Ardin, A. C. ; Grönroos, L. ; Alaluusua, S. ; Ooshima, T. ; Nakano, Michiyo. / Correlation of biological properties with glucan-binding protein B expression profile in Streptococcus mutans clinical isolates. In: Archives of Oral Biology. 2011 ; Vol. 56, No. 3. pp. 258-263.
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