TY - JOUR
T1 - Correlation between the number of apoptotic cells and expression of the apoptosis‐related antigens Fas, Ley and bcl‐2 protein in non‐Hodgkin's lymphomas
AU - Aftabuddin, Mohammad
AU - Yamadori, Ichiro
AU - Yoshino, Tadashi
AU - Kondo, Eisaku
AU - Akagi, Tadaatsu
PY - 1995/6
Y1 - 1995/6
N2 - The relationship between the number of apoptotic cells and the expression of apoptosis‐related antigens was examined In 56 cases of non‐Hodgkin's lymphomas and in 10 cases of reactive hyperplastic lymph nodes (RHL). Apoptosis was visually quantified by the in situ end‐labeling (ISEL) method, and the expression of Fas, Ley antigens and bcl‐2 protein was examined by Immunohistochemistry. The expression of Ley antigen was observed in germinal centers of RHL and 45% of non‐Hodgkin's lymphomas. The apoptotic cell count (AC) in follicular lymphomas was significantly less than that in diffuse lymphomas. The distribution pattern of apoptotic cells In follicular lymphomas was inverse to that in RHL. In follicular lymphomas, AC was lower in follicles than in inter‐follicular areas. In contrast, AC was higher in follicles than in Interfollicular areas in RHL. Ley antigen‐positive lymphomas showed a significantly higher AC than the negative cases. The Fas antigen‐positive lymphomas showed a higher AC than the negative cases. However, AC in bcl‐2 protein‐positive and negative cases was not significantly different. These results suggest that Ley and Fas antigens appear to be involved in the apoptotic tendency of tumor cells in non‐Hodgkin's lymphomas, whereas bcl‐2 does not necessarily.
AB - The relationship between the number of apoptotic cells and the expression of apoptosis‐related antigens was examined In 56 cases of non‐Hodgkin's lymphomas and in 10 cases of reactive hyperplastic lymph nodes (RHL). Apoptosis was visually quantified by the in situ end‐labeling (ISEL) method, and the expression of Fas, Ley antigens and bcl‐2 protein was examined by Immunohistochemistry. The expression of Ley antigen was observed in germinal centers of RHL and 45% of non‐Hodgkin's lymphomas. The apoptotic cell count (AC) in follicular lymphomas was significantly less than that in diffuse lymphomas. The distribution pattern of apoptotic cells In follicular lymphomas was inverse to that in RHL. In follicular lymphomas, AC was lower in follicles than in inter‐follicular areas. In contrast, AC was higher in follicles than in Interfollicular areas in RHL. Ley antigen‐positive lymphomas showed a significantly higher AC than the negative cases. The Fas antigen‐positive lymphomas showed a higher AC than the negative cases. However, AC in bcl‐2 protein‐positive and negative cases was not significantly different. These results suggest that Ley and Fas antigens appear to be involved in the apoptotic tendency of tumor cells in non‐Hodgkin's lymphomas, whereas bcl‐2 does not necessarily.
KW - Fas
KW - Le
KW - bcl‐2 protein
KW - in situ end‐labeling
KW - non‐Hodgkin's lymphoma
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U2 - 10.1111/j.1440-1827.1995.tb03479.x
DO - 10.1111/j.1440-1827.1995.tb03479.x
M3 - Article
C2 - 7581933
AN - SCOPUS:0029031068
VL - 45
SP - 422
EP - 429
JO - Acta Pathologica Japonica
JF - Acta Pathologica Japonica
SN - 1320-5463
IS - 6
ER -