Correlation between HPV positivity and state of the p53 gene in cervical carcinoma cell lines

Tsuyoshi Iwasaka, Mamoru Oh-Uchida, Norihito Matsuo, Masatoshi Yokoyama, Kouichi Fukuda, Kouichi Hara, Kouzo Fukuyama, Katsuji Hori, Hajime Sugimori

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

Human papillomavirus (HPV) infection is clearly associated with cervical carcinomas, yet it is also true that there are cervical carcinomas in which HPV DNA is absent. We examined eight established cell lines derived from cervical carcinomas for the presence of mutations of the p53 antioncogene in relation to the presence of HPV DNA sequences. Of these eight cell lines, seven were positive for HPV DNA and the remaining one was negative for HPV DNA. Single-strand conformation polymorphism analyses revealed a point mutation of the p53 gene in the cell line in which HPV DNA was absent. Sequencing analysis revealed a single-base mutation at codon 273 from CGT to CAT(Arg → His) and immunocytochemical studies provided evidence that the p53 protein was overexpressed in this cell line. Our observations suggest that the loss of normal p53 gene function may be linked to the oncogenesis of cervical carcinoma.

Original languageEnglish
Pages (from-to)104-109
Number of pages6
JournalGynecologic Oncology
Volume48
Issue number1
DOIs
Publication statusPublished - Jan 1993
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Obstetrics and Gynaecology

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