TY - JOUR
T1 - Copy number abnormality of acute lymphoblastic leukemia cell lines based on their genetic subtypes
AU - Tomoyasu, Chihiro
AU - Imamura, Toshihiko
AU - Tomii, Toshihiro
AU - Yano, Mio
AU - Asai, Daisuke
AU - Goto, Hiroaki
AU - Shimada, Akira
AU - Sanada, Masashi
AU - Iwamoto, Shotaro
AU - Takita, Junko
AU - Minegishi, Masayoshi
AU - Inukai, Takeshi
AU - Sugita, Kanji
AU - Hosoi, Hajime
N1 - Funding Information:
SU-Ph2 was established at Kinki University School of Medicine, Osaka, and provided in 2010 (Dr. Y. Maeda). TCCY was established at Tochigi Cancer Center and provided in 2011 (Dr. Y. Sato). HALO1 and SK9 were established at Tokyo Medical University, Tokyo, and provided in 1997 (Dr. T. Look in Dana-Farber Cancer Institute, Boston, MA) and in 2012 (Dr. S. Okabe), respectively. Endokun was established at Iwate Medical University, Morioka, and provided in 1997 (Dr. M. Endo). Kasumi2 established at Hiroshima University, Hiroshima, and provided in 2010 (Dr. T. Inaba). The authors declare that they have no conflict of interest. A summary of relevant information will be published with the manuscript.
Publisher Copyright:
© 2018, The Japanese Society of Hematology.
PY - 2018/9/1
Y1 - 2018/9/1
N2 - In this study, we performed genetic analysis of 83 B cell precursor acute lymphoblastic leukemia (B-ALL) cell lines. First, we performed multiplex ligation-dependent probe amplification analysis to identify copy number abnormalities (CNAs) in eight genes associated with B-ALL according to genetic subtype. In Ph+ B-ALL cell lines, the frequencies of IKZF1, CDKN2A/2B, BTG1, and PAX5 deletion were significantly higher than those in Ph− B-ALL cell lines. The frequency of CDKN2A/2B deletion in KMT2A rearranged cell lines was significantly lower than that in non-KMT2A rearranged cell lines. These findings suggest that CNAs are correlated with genetic subtype in B-ALL cell lines. In addition, we determined that three B-other ALL cell lines had IKZF1 deletions (YCUB-5, KOPN49, and KOPN75); we therefore performed comprehensive genetic analysis of these cell lines. YCUB-5, KOPN49, and KOPN75 had P2RY8-CRLF2, IgH-CRLF2, and PAX5-ETV6 fusions, respectively. Moreover, targeted capture sequencing revealed that YCUB-5 had JAK2 R683I and KRAS G12D, and KOPN49 had JAK2 R683G and KRAS G13D mutations. These data may contribute to progress in the field of leukemia research.
AB - In this study, we performed genetic analysis of 83 B cell precursor acute lymphoblastic leukemia (B-ALL) cell lines. First, we performed multiplex ligation-dependent probe amplification analysis to identify copy number abnormalities (CNAs) in eight genes associated with B-ALL according to genetic subtype. In Ph+ B-ALL cell lines, the frequencies of IKZF1, CDKN2A/2B, BTG1, and PAX5 deletion were significantly higher than those in Ph− B-ALL cell lines. The frequency of CDKN2A/2B deletion in KMT2A rearranged cell lines was significantly lower than that in non-KMT2A rearranged cell lines. These findings suggest that CNAs are correlated with genetic subtype in B-ALL cell lines. In addition, we determined that three B-other ALL cell lines had IKZF1 deletions (YCUB-5, KOPN49, and KOPN75); we therefore performed comprehensive genetic analysis of these cell lines. YCUB-5, KOPN49, and KOPN75 had P2RY8-CRLF2, IgH-CRLF2, and PAX5-ETV6 fusions, respectively. Moreover, targeted capture sequencing revealed that YCUB-5 had JAK2 R683I and KRAS G12D, and KOPN49 had JAK2 R683G and KRAS G13D mutations. These data may contribute to progress in the field of leukemia research.
KW - Acute lymphoblastic leukemia cell line
KW - BTG1
KW - CDKN2A
KW - CDKN2B
KW - Copy number abnormality
KW - IKZF1
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U2 - 10.1007/s12185-018-2474-7
DO - 10.1007/s12185-018-2474-7
M3 - Article
C2 - 29786757
AN - SCOPUS:85047187219
SN - 0925-5710
VL - 108
SP - 312
EP - 318
JO - International Journal of Hematology
JF - International Journal of Hematology
IS - 3
ER -