Coordination of motilin and ghrelin regulates the migrating motor complex of gastrointestinal motility in Suncus murinus

Anupom Mondal, Zuoyun Xie, Yuki Miyano, Chihiro Tsutsui, Ichiro Sakata, Yoichi Kawamoto, Sayaka Aizawa, Toru Tanaka, Sen Ichi Oda, Takafumi Sakai

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Motilin and ghrelin are the gastrointestinal (GI) hormones released in a fasting state to stimulate the GI motility of the migrating motor complex (MMC). We focused on coordination of the ghrelin/motilin family in gastric contraction in vivo and in vitro using the house musk shrew (Suncus murinus), a ghrelin- and motilin-producing mammal. To measure the contractile activity of the stomach in vivo, we recorded GI contractions either in the free-moving conscious or anesthetized S. murinus and examined the effects of administration of motilin and/or ghrelin on spontaneous MMC in the fasting state. In the in vitro study, we also studied the coordinative effect of these hormones on the isolated stomach using an organ bath. In the fasting state, phase I, II, and III contractions were clearly recorded in the gastric body (as observed in humans and dogs). Intravenous infusion of ghrelin stimulated gastric contraction in the latter half of phase I and in the phase II in a dose-dependent manner. Continuous intravenous infusion of ghrelin antagonist (D-Lys3- GHRP6) significantly suppressed spontaneous phase II contractions and prolonged the time of occurrence of the peak of phase III contractions. However, intravenous infusion of motilin antagonist (MA-2029) did not inhibit phase II contractions but delayed the occurrence of phase III contractions of the MMC. In the in vitro study, even though a high dose of ghrelin did not stimulate contraction of stomach preparations, ghrelin administration (10 -10-10 -7 M) with pretreatment of a low dose of motilin (10 -10 M) induced gastric contraction in a dose-dependent manner. Pretreatment with 10 -8 M ghrelin enhanced motilin-stimulated gastric contractions by 10 times. The interrelation of these peptides was also demonstrated in the anesthetized S. murinus. The results suggest that ghrelin is important for the phase II contraction and that coordination of motilin and ghrelin are necessary to initiate phase III contraction of the MMC.

Original languageEnglish
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume302
Issue number10
DOIs
Publication statusPublished - May 15 2012
Externally publishedYes

Fingerprint

Migrating Myoelectric Complexes
Motilin
Ghrelin
Gastrointestinal Motility
Stomach
Intravenous Infusions
Fasting
Shrews
Gastrointestinal Hormones
Baths
Mammals

Keywords

  • Migrating motor complex

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology (medical)
  • Physiology
  • Hepatology

Cite this

Coordination of motilin and ghrelin regulates the migrating motor complex of gastrointestinal motility in Suncus murinus. / Mondal, Anupom; Xie, Zuoyun; Miyano, Yuki; Tsutsui, Chihiro; Sakata, Ichiro; Kawamoto, Yoichi; Aizawa, Sayaka; Tanaka, Toru; Oda, Sen Ichi; Sakai, Takafumi.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 302, No. 10, 15.05.2012.

Research output: Contribution to journalArticle

Mondal, Anupom ; Xie, Zuoyun ; Miyano, Yuki ; Tsutsui, Chihiro ; Sakata, Ichiro ; Kawamoto, Yoichi ; Aizawa, Sayaka ; Tanaka, Toru ; Oda, Sen Ichi ; Sakai, Takafumi. / Coordination of motilin and ghrelin regulates the migrating motor complex of gastrointestinal motility in Suncus murinus. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2012 ; Vol. 302, No. 10.
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AU - Sakata, Ichiro

AU - Kawamoto, Yoichi

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