Coordinated expression patterns of genes encoding cell wall modifying enzymes during ripening in distinct anatomical tissue regions of the fig (Ficus carica L.) fruit

Willis Omondi Owino, Ryohei Nakano, Yasutaka Kubo, Akitsugu Inaba

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Fig (Ficus carica L.) fruit undergoes rapid softening during ripening, a process that involves changes in the solubility and molecular size of the cell wall polysaccharides in both the receptacle and drupelet tissues. In this study we report on the isolation and characterization of the expression patterns of eleven genes encoding a collection of cell wall modifying enzymes during ripening of the fig fruit. The genes studied include those encoding endo-polygalacturonases (PGs), endo-glucanases (EGases), β-galactosidases (β-Gals), xyloglucan endotransglycosylases (XTHs,), expansins (Exps) and α-arabinofuranosidases (Arabfs). The Northern analysis data revealed that the expression patterns of these genes in both tissues during fig fruit ripening could be divided into three categories. The Fc-Pg1, Fc-Pg2, Fc-Cel1, Fc-Cel2 and Fc-Gal2 transcripts showed increased accumulation from the ripening onset to the over ripe stage and were clustered into group (I). The Fc-Exp1, Fc-Gal1, Fc-Arabf1, Fc-XTH1 and Fc-XTH2 transcripts accumulated at the immature stage and were clustered into group (II). The Fc-XTH3 transcript accumulated only at the over ripe stage and was the only member of group (III). Other than Fc-PG2 which was specific to the drupelet tissue, all the other genes were detected in both the receptacle and the drupelets tissue albeit at differing stages of ripening and with different intensity of accumulation. This study suggests that the gene products of the 11 isolated cDNAs putatively encoding cell wall related enzymes are coordinated both in time and amount during fig fruit development and ripening and act together in an interdependent way to achieve softening.

Original languageEnglish
Pages (from-to)253-261
Number of pages9
JournalPostharvest Biology and Technology
Volume32
Issue number3
DOIs
Publication statusPublished - Jun 2004

Fingerprint

Carica
Ficus
Ficus carica
figs
Cell Wall
Fruit
ripening
cell walls
Gene Expression
fruits
Enzymes
enzymes
genes
Genes
Galactosidases
Polygalacturonase
alpha-N-arabinofuranosidase
galactosidases
expansins
xyloglucans

Keywords

  • Cell wall disassembly (enzymes, cDNAs)
  • Fig fruit
  • Ripening
  • Softening

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Food Science
  • Horticulture

Cite this

Coordinated expression patterns of genes encoding cell wall modifying enzymes during ripening in distinct anatomical tissue regions of the fig (Ficus carica L.) fruit. / Owino, Willis Omondi; Nakano, Ryohei; Kubo, Yasutaka; Inaba, Akitsugu.

In: Postharvest Biology and Technology, Vol. 32, No. 3, 06.2004, p. 253-261.

Research output: Contribution to journalArticle

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