Coordinated change between complement C1s production and chondrocyte differentiation in vitro

Koichi Nakagawa, Hisako Sakiyama, Takeshi Fukazawa, Misako Matsumoto, Masaharu Takigawa, Toru Toyoguchi, Hideshige Moriya

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Abstract

In vitro synthesis of the first component of complement C1s was examined by using hamster epiphyseal chondrocytes (HAC) and human chondrosarcoma cell line HCS-2/8. Hamster and human C1s produced by the cells were quantified by immunoblotting and sandwich enzyme-linked immunosorbent assay (ELISA), respectively. It was possible to measure active and inactive C1s by sandwich ELISA, when we used anti-human C1s monoclonal antibodies, M241 recognizing only active C1s, and M365 and M81 recognizing both active and inactive C1s. Approximately 40% of C1s secreted from HCS-2/8 was found to be activated in the culture medium, whereas C1s from HAC was not. C1s production increased in accordance with chondrocyte differentiation induced by ascorbic acid. In contrast, transforming growth factor-beta1 and basic fibroblast growth factor, which inhibited differentiation, suppressed C1s production. These results confirmed our previous observation showing that C1s synthesis increased with differentiation into hypertrophic chondrocytes in vivo.

Original languageEnglish
Pages (from-to)299-305
Number of pages7
JournalCell and Tissue Research
Volume289
Issue number2
DOIs
Publication statusPublished - 1997

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Keywords

  • Ascorbic acid
  • Cell culture
  • Chondrocytes
  • Complement C1s
  • Differentiation
  • Human
  • Sandwich ELISA
  • Syrian hamster

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Nakagawa, K., Sakiyama, H., Fukazawa, T., Matsumoto, M., Takigawa, M., Toyoguchi, T., & Moriya, H. (1997). Coordinated change between complement C1s production and chondrocyte differentiation in vitro. Cell and Tissue Research, 289(2), 299-305. https://doi.org/10.1007/s004410050876