Cooperative function of PLDδ and PLDα1 in abscisic acid-induced stomatal closure in arabidopsis

Misugi Uraji, Takeshi Katagiri, Eiji Okuma, Wenxiu Ye, Mohammad Anowar Hossain, Choji Masuda, Aya Miura, Yoshimasa Nakamura, Izumi Mori, Kazuo Shinozaki, Yoshiyuki Murata

Research output: Contribution to journalArticle

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Abstract

Phospholipase D (PLD) is involved in responses to abiotic stress and abscisic acid (ABA) signaling. To investigate the roles of two Arabidopsis (Arabidopsis thaliana) PLDs, PLDα1 and PLDδ, in ABA signaling in guard cells, we analyzed ABA responses in guard cells using Arabidopsis wild type, pldα1 and pldδ single mutants, and a pldα1 pldδ double mutant. ABA-induced stomatal closure was suppressed in the pldα1 pldδ double mutant but not in the pld single mutants. The pldα1 and pldδ mutations reduced ABAinduced phosphatidic acid production in epidermal tissues. Expression of either PLDα1 or PLDδ complemented the double mutant stomatal phenotype. ABA-induced stomatal closure in both pldα1 and pldδ single mutants was inhibited by a PLD inhibitor (1-butanol), suggesting that both PLDα1 and PLDδ function in ABA-induced stomatal closure. During ABA-induced stomatal closure, wild-type guard cells accumulate reactive oxygen species and nitric oxide and undergo cytosolic alkalization, but these changes are reduced in guard cells of the pld*alpha;1 pldδ double mutant. Inward-rectifying K+ channel currents of guard cells were inhibited by ABA in the wild type but not in the pldα1 pldδ double mutant. ABA inhibited stomatal opening in the wild type and the pldδ mutant but not in the pldα1 mutant. In wild-type rosette leaves, ABA significantly increased PLDδ transcript levels but did not change PLDα1 transcript levels. Furthermore, the pldα1 and pldδ mutations mitigated ABA inhibition of seed germination. These results suggest that PLDα1 and PLDδ cooperate in ABA signaling in guard cells but that their functions do not completely overlap.

Original languageEnglish
Pages (from-to)450-460
Number of pages11
JournalPlant Physiology
Volume159
Issue number1
DOIs
Publication statusPublished - May 2012

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Phospholipase D
phospholipase D
Abscisic Acid
Arabidopsis
cooperatives
abscisic acid
guard cells
mutants
mutation
alkalinization
Phosphatidic Acids
1-Butanol
Mutation
potassium channels
butanol
Germination
abiotic stress
nitric oxide
reactive oxygen species
Reactive Oxygen Species

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

Cooperative function of PLDδ and PLDα1 in abscisic acid-induced stomatal closure in arabidopsis. / Uraji, Misugi; Katagiri, Takeshi; Okuma, Eiji; Ye, Wenxiu; Hossain, Mohammad Anowar; Masuda, Choji; Miura, Aya; Nakamura, Yoshimasa; Mori, Izumi; Shinozaki, Kazuo; Murata, Yoshiyuki.

In: Plant Physiology, Vol. 159, No. 1, 05.2012, p. 450-460.

Research output: Contribution to journalArticle

Uraji, M, Katagiri, T, Okuma, E, Ye, W, Hossain, MA, Masuda, C, Miura, A, Nakamura, Y, Mori, I, Shinozaki, K & Murata, Y 2012, 'Cooperative function of PLDδ and PLDα1 in abscisic acid-induced stomatal closure in arabidopsis', Plant Physiology, vol. 159, no. 1, pp. 450-460. https://doi.org/10.1104/pp.112.195578
Uraji, Misugi ; Katagiri, Takeshi ; Okuma, Eiji ; Ye, Wenxiu ; Hossain, Mohammad Anowar ; Masuda, Choji ; Miura, Aya ; Nakamura, Yoshimasa ; Mori, Izumi ; Shinozaki, Kazuo ; Murata, Yoshiyuki. / Cooperative function of PLDδ and PLDα1 in abscisic acid-induced stomatal closure in arabidopsis. In: Plant Physiology. 2012 ; Vol. 159, No. 1. pp. 450-460.
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