Converting a light-driven proton pump into a light-gated proton channel

Keiichi Inoue, Takashi Tsukamoto, Kazumi Shimono, Yuto Suzuki, Seiji Miyauchi, Shigehiko Hayashi, Hideki Kandori, Yuki Sudo

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

There are two types of membrane-embedded ion transport machineries in nature. The ion pumps generate electrochemical potential by energy-coupled active ion transportation, while the ion channels produce action potential by stimulus-dependent passive ion transportation. About 80% of the amino acid residues of the light-driven proton pump archaerhodopsin-3 (AR3) and the light-gated cation channel channelrhodopsin (ChR) differ although they share the close similarity in architecture. Therefore, the question arises: How can these proteins function differently? The absorption maxima of ion pumps are red-shifted about 30-100 nm compared with ChRs, implying a structural difference in the retinal binding cavity. To modify the cavity, a blue-shifted AR3 named AR3-T was produced by replacing three residues located around the retinal (i.e., M128A, G132V, and A225T). AR3-T showed an inward H+ flux across the membrane, raising the possibility that it works as an inward H+ pump or an H+ channel. Electrophysiological experiments showed that the reverse membrane potential was nearly zero, indicating light-gated ion channeling activity of AR3-T. Spectroscopic characterization of AR3-T revealed similar photochemical properties to some of ChRs, including an all-trans retinal configuration, a strong hydrogen bond between the protonated retinal Schiff base and its counterion, and a slow photocycle. From these results, we concluded that the functional determinant in the H+ transporters is localized at the center of the membrane-spanning domain, but not in the cytoplasmic and extracellular domains.

Original languageEnglish
Pages (from-to)3291-3299
Number of pages9
JournalJournal of the American Chemical Society
Volume137
Issue number9
DOIs
Publication statusPublished - Feb 2015

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Fingerprint Dive into the research topics of 'Converting a light-driven proton pump into a light-gated proton channel'. Together they form a unique fingerprint.

  • Cite this

    Inoue, K., Tsukamoto, T., Shimono, K., Suzuki, Y., Miyauchi, S., Hayashi, S., Kandori, H., & Sudo, Y. (2015). Converting a light-driven proton pump into a light-gated proton channel. Journal of the American Chemical Society, 137(9), 3291-3299. https://doi.org/10.1021/ja511788f