Convergent loss of awn in two cultivated rice species Oryza sativa and Oryza glaberrima is caused by mutations in different loci

Tomoyuki Furuta, Norio Komeda, Kenji Asano, Kanako Uehara, Rico Gamuyao, Rosalyn B. Angeles-Shim, Keisuke Nagai, Kazuyuki Doi, Diane R. Wang, Hideshi Yasui, Atsushi Yoshimura, Jianzhong Wu, Susan R. McCouch, Motoyuki Ashikari

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A long awn is one of the distinct morphological features of wild rice species. This organ is thought to aid in seed dispersal and prevent predation by animals. Most cultivated varieties of Oryza sativa and Oryza glaberrima, however, have lost the ability to form long awns. The causal genetic factors responsible for the loss of awn in these two rice species remain largely unknown. Here, we evaluated three sets of chromosome segment substitution lines (CSSLs) in a common O. sativa genetic background (cv. Koshihikari) that harbor genomic fragments from Oryza nivara, Oryza rufipogon, and Oryza glaberrima donors. Phenotypic analyses of these libraries revealed the existence of three genes, Regulator of Awn Elongation 1 (RAE1), RAE2, and RAE3, involved in the loss of long awns in cultivated rice. Donor segments at two of these genes, RAE1 and RAE2, induced long awn formation in the CSSLs whereas an O. sativa segment at RAE3 induced long awn formation in O. glaberrima. These results suggest that the two cultivated rice species, O. sativa and O. glaberrima, have taken independent paths to become awnless.

Original languageEnglish
Pages (from-to)2267-2274
Number of pages8
JournalG3: Genes, Genomes, Genetics
Volume5
Issue number11
DOIs
Publication statusPublished - Jan 1 2015
Externally publishedYes

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Keywords

  • African rice
  • Asian rice
  • Awn
  • CSSLs
  • Domestication

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Furuta, T., Komeda, N., Asano, K., Uehara, K., Gamuyao, R., Angeles-Shim, R. B., Nagai, K., Doi, K., Wang, D. R., Yasui, H., Yoshimura, A., Wu, J., McCouch, S. R., & Ashikari, M. (2015). Convergent loss of awn in two cultivated rice species Oryza sativa and Oryza glaberrima is caused by mutations in different loci. G3: Genes, Genomes, Genetics, 5(11), 2267-2274. https://doi.org/10.1534/g3.115.020834