Control of the Water Transport Activity of Barley HvTIP3;1 Specifically Expressed in Seeds

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Tonoplast intrinsic proteins (TIPs) are involved in the transport and storage of water, and control intracellular osmotic pressure by transporting material related to the water potential of cells. In the present study, we focused on HvTIP3;1 during the periods of seed development and desiccation in barley. HvTIP3;1 was specifically expressed in seeds. An immunochemical analysis showed that HvTIP3;1 strongly accumulated in the aleurone layers and outer layers of barley seeds. The water transport activities of HvTIP3;1 and HvTIP1;2, which also accumulated in seeds, were measured in the heterologous expression system of Xenopus oocytes. When they were expressed individually, HvTIP1;2 transported water, whereas HvTIP3;1 did not. However, HvTIP3;1 exhibited water transport activity when co-expressed with HvTIP1;2 in oocytes, and this activity was higher than when HvTIP1;2 was expressed alone. This is the first report to demonstrate that the water permeability of a TIP aquaporin was activated when co-expressed with another TIP. The split-yellow fluorescent protein (YFP) system in onion cells revealed that HvTIP3;1 interacted with HvTIP1;2 to form a heterotetramer in plants. These results suggest that HvTIP3;1 functions as an active water channel to regulate water movement through tissues during the periods of seed development and desiccation.

Original languageEnglish
Pages (from-to)1831-1840
Number of pages10
JournalPlant and Cell Physiology
Volume56
Issue number9
DOIs
Publication statusPublished - 2015

Fingerprint

Hordeum
Seeds
barley
Water
seeds
tonoplast
Desiccation
Aquaporins
water
desiccation (plant physiology)
Oocytes
seed development
Proteins
oocytes
Water Movements
Onions
Osmotic Pressure
aleurone layer
Xenopus
aquaporins

Keywords

  • Aquaporin
  • Barley
  • Seed
  • TIP
  • Water transport activity

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Control of the Water Transport Activity of Barley HvTIP3;1 Specifically Expressed in Seeds. / Utsugi, Shigeko; Shibasaka, Mineo; Maekawa, Masahiko; Katsuhara, Maki.

In: Plant and Cell Physiology, Vol. 56, No. 9, 2015, p. 1831-1840.

Research output: Contribution to journalArticle

@article{f6222e38cc424129943a2fc53a4d863b,
title = "Control of the Water Transport Activity of Barley HvTIP3;1 Specifically Expressed in Seeds",
abstract = "Tonoplast intrinsic proteins (TIPs) are involved in the transport and storage of water, and control intracellular osmotic pressure by transporting material related to the water potential of cells. In the present study, we focused on HvTIP3;1 during the periods of seed development and desiccation in barley. HvTIP3;1 was specifically expressed in seeds. An immunochemical analysis showed that HvTIP3;1 strongly accumulated in the aleurone layers and outer layers of barley seeds. The water transport activities of HvTIP3;1 and HvTIP1;2, which also accumulated in seeds, were measured in the heterologous expression system of Xenopus oocytes. When they were expressed individually, HvTIP1;2 transported water, whereas HvTIP3;1 did not. However, HvTIP3;1 exhibited water transport activity when co-expressed with HvTIP1;2 in oocytes, and this activity was higher than when HvTIP1;2 was expressed alone. This is the first report to demonstrate that the water permeability of a TIP aquaporin was activated when co-expressed with another TIP. The split-yellow fluorescent protein (YFP) system in onion cells revealed that HvTIP3;1 interacted with HvTIP1;2 to form a heterotetramer in plants. These results suggest that HvTIP3;1 functions as an active water channel to regulate water movement through tissues during the periods of seed development and desiccation.",
keywords = "Aquaporin, Barley, Seed, TIP, Water transport activity",
author = "Shigeko Utsugi and Mineo Shibasaka and Masahiko Maekawa and Maki Katsuhara",
year = "2015",
doi = "10.1093/pcp/pcv104",
language = "English",
volume = "56",
pages = "1831--1840",
journal = "Plant and Cell Physiology",
issn = "0032-0781",
publisher = "Oxford University Press",
number = "9",

}

TY - JOUR

T1 - Control of the Water Transport Activity of Barley HvTIP3;1 Specifically Expressed in Seeds

AU - Utsugi, Shigeko

AU - Shibasaka, Mineo

AU - Maekawa, Masahiko

AU - Katsuhara, Maki

PY - 2015

Y1 - 2015

N2 - Tonoplast intrinsic proteins (TIPs) are involved in the transport and storage of water, and control intracellular osmotic pressure by transporting material related to the water potential of cells. In the present study, we focused on HvTIP3;1 during the periods of seed development and desiccation in barley. HvTIP3;1 was specifically expressed in seeds. An immunochemical analysis showed that HvTIP3;1 strongly accumulated in the aleurone layers and outer layers of barley seeds. The water transport activities of HvTIP3;1 and HvTIP1;2, which also accumulated in seeds, were measured in the heterologous expression system of Xenopus oocytes. When they were expressed individually, HvTIP1;2 transported water, whereas HvTIP3;1 did not. However, HvTIP3;1 exhibited water transport activity when co-expressed with HvTIP1;2 in oocytes, and this activity was higher than when HvTIP1;2 was expressed alone. This is the first report to demonstrate that the water permeability of a TIP aquaporin was activated when co-expressed with another TIP. The split-yellow fluorescent protein (YFP) system in onion cells revealed that HvTIP3;1 interacted with HvTIP1;2 to form a heterotetramer in plants. These results suggest that HvTIP3;1 functions as an active water channel to regulate water movement through tissues during the periods of seed development and desiccation.

AB - Tonoplast intrinsic proteins (TIPs) are involved in the transport and storage of water, and control intracellular osmotic pressure by transporting material related to the water potential of cells. In the present study, we focused on HvTIP3;1 during the periods of seed development and desiccation in barley. HvTIP3;1 was specifically expressed in seeds. An immunochemical analysis showed that HvTIP3;1 strongly accumulated in the aleurone layers and outer layers of barley seeds. The water transport activities of HvTIP3;1 and HvTIP1;2, which also accumulated in seeds, were measured in the heterologous expression system of Xenopus oocytes. When they were expressed individually, HvTIP1;2 transported water, whereas HvTIP3;1 did not. However, HvTIP3;1 exhibited water transport activity when co-expressed with HvTIP1;2 in oocytes, and this activity was higher than when HvTIP1;2 was expressed alone. This is the first report to demonstrate that the water permeability of a TIP aquaporin was activated when co-expressed with another TIP. The split-yellow fluorescent protein (YFP) system in onion cells revealed that HvTIP3;1 interacted with HvTIP1;2 to form a heterotetramer in plants. These results suggest that HvTIP3;1 functions as an active water channel to regulate water movement through tissues during the periods of seed development and desiccation.

KW - Aquaporin

KW - Barley

KW - Seed

KW - TIP

KW - Water transport activity

UR - http://www.scopus.com/inward/record.url?scp=84941248642&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84941248642&partnerID=8YFLogxK

U2 - 10.1093/pcp/pcv104

DO - 10.1093/pcp/pcv104

M3 - Article

VL - 56

SP - 1831

EP - 1840

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

IS - 9

ER -