Control of seed dormancy and germination by DOG1-AHG1 PP2C phosphatase complex via binding to heme

Noriyuki Nishimura, Wataru Tsuchiya, James J. Moresco, Yuki Hayashi, Kouji Satoh, Nahomi Kaiwa, Tomoko Irisa, Toshinori Kinoshita, Julian I. Schroeder, John R. Yates, Takashi Hirayama, Toshimasa Yamazaki

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Abscisic acid (ABA) regulates abiotic stress and developmental responses including regulation of seed dormancy to prevent seeds from germinating under unfavorable environmental conditions. ABA HYPERSENSITIVE GERMINATION1 (AHG1) encoding a type 2C protein phosphatase (PP2C) is a central negative regulator of ABA response in germination; however, the molecular function and regulation of AHG1 remain elusive. Here we report that AHG1 interacts with DELAY OF GERMINATION1 (DOG1), which is a pivotal positive regulator in seed dormancy. DOG1 acts upstream of AHG1 and impairs the PP2C activity of AHG1 in vitro. Furthermore, DOG1 has the ability to bind heme. Binding of DOG1 to AHG1 and heme are independent processes, but both are essential for DOG1 function in vivo. Our study demonstrates that AHG1 and DOG1 constitute an important regulatory system for seed dormancy and germination by integrating multiple environmental signals, in parallel with the PYL/RCAR ABA receptor-mediated regulatory system.

Original languageEnglish
Article number2132
JournalNature Communications
Volume9
Issue number1
DOIs
Publication statusPublished - Dec 1 2018

Fingerprint

abscisic acid
Plant Dormancy
germination
Abscisic Acid
phosphatases
Germination
Heme
Phosphoric Monoester Hydrolases
Seed
seeds
regulators
Phosphoprotein Phosphatases
upstream
Seeds
coding
proteins

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Nishimura, N., Tsuchiya, W., Moresco, J. J., Hayashi, Y., Satoh, K., Kaiwa, N., ... Yamazaki, T. (2018). Control of seed dormancy and germination by DOG1-AHG1 PP2C phosphatase complex via binding to heme. Nature Communications, 9(1), [2132]. https://doi.org/10.1038/s41467-018-04437-9

Control of seed dormancy and germination by DOG1-AHG1 PP2C phosphatase complex via binding to heme. / Nishimura, Noriyuki; Tsuchiya, Wataru; Moresco, James J.; Hayashi, Yuki; Satoh, Kouji; Kaiwa, Nahomi; Irisa, Tomoko; Kinoshita, Toshinori; Schroeder, Julian I.; Yates, John R.; Hirayama, Takashi; Yamazaki, Toshimasa.

In: Nature Communications, Vol. 9, No. 1, 2132, 01.12.2018.

Research output: Contribution to journalArticle

Nishimura, N, Tsuchiya, W, Moresco, JJ, Hayashi, Y, Satoh, K, Kaiwa, N, Irisa, T, Kinoshita, T, Schroeder, JI, Yates, JR, Hirayama, T & Yamazaki, T 2018, 'Control of seed dormancy and germination by DOG1-AHG1 PP2C phosphatase complex via binding to heme', Nature Communications, vol. 9, no. 1, 2132. https://doi.org/10.1038/s41467-018-04437-9
Nishimura, Noriyuki ; Tsuchiya, Wataru ; Moresco, James J. ; Hayashi, Yuki ; Satoh, Kouji ; Kaiwa, Nahomi ; Irisa, Tomoko ; Kinoshita, Toshinori ; Schroeder, Julian I. ; Yates, John R. ; Hirayama, Takashi ; Yamazaki, Toshimasa. / Control of seed dormancy and germination by DOG1-AHG1 PP2C phosphatase complex via binding to heme. In: Nature Communications. 2018 ; Vol. 9, No. 1.
@article{9a3559c616d64445a5f013749bb3f2bf,
title = "Control of seed dormancy and germination by DOG1-AHG1 PP2C phosphatase complex via binding to heme",
abstract = "Abscisic acid (ABA) regulates abiotic stress and developmental responses including regulation of seed dormancy to prevent seeds from germinating under unfavorable environmental conditions. ABA HYPERSENSITIVE GERMINATION1 (AHG1) encoding a type 2C protein phosphatase (PP2C) is a central negative regulator of ABA response in germination; however, the molecular function and regulation of AHG1 remain elusive. Here we report that AHG1 interacts with DELAY OF GERMINATION1 (DOG1), which is a pivotal positive regulator in seed dormancy. DOG1 acts upstream of AHG1 and impairs the PP2C activity of AHG1 in vitro. Furthermore, DOG1 has the ability to bind heme. Binding of DOG1 to AHG1 and heme are independent processes, but both are essential for DOG1 function in vivo. Our study demonstrates that AHG1 and DOG1 constitute an important regulatory system for seed dormancy and germination by integrating multiple environmental signals, in parallel with the PYL/RCAR ABA receptor-mediated regulatory system.",
author = "Noriyuki Nishimura and Wataru Tsuchiya and Moresco, {James J.} and Yuki Hayashi and Kouji Satoh and Nahomi Kaiwa and Tomoko Irisa and Toshinori Kinoshita and Schroeder, {Julian I.} and Yates, {John R.} and Takashi Hirayama and Toshimasa Yamazaki",
year = "2018",
month = "12",
day = "1",
doi = "10.1038/s41467-018-04437-9",
language = "English",
volume = "9",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Control of seed dormancy and germination by DOG1-AHG1 PP2C phosphatase complex via binding to heme

AU - Nishimura, Noriyuki

AU - Tsuchiya, Wataru

AU - Moresco, James J.

AU - Hayashi, Yuki

AU - Satoh, Kouji

AU - Kaiwa, Nahomi

AU - Irisa, Tomoko

AU - Kinoshita, Toshinori

AU - Schroeder, Julian I.

AU - Yates, John R.

AU - Hirayama, Takashi

AU - Yamazaki, Toshimasa

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Abscisic acid (ABA) regulates abiotic stress and developmental responses including regulation of seed dormancy to prevent seeds from germinating under unfavorable environmental conditions. ABA HYPERSENSITIVE GERMINATION1 (AHG1) encoding a type 2C protein phosphatase (PP2C) is a central negative regulator of ABA response in germination; however, the molecular function and regulation of AHG1 remain elusive. Here we report that AHG1 interacts with DELAY OF GERMINATION1 (DOG1), which is a pivotal positive regulator in seed dormancy. DOG1 acts upstream of AHG1 and impairs the PP2C activity of AHG1 in vitro. Furthermore, DOG1 has the ability to bind heme. Binding of DOG1 to AHG1 and heme are independent processes, but both are essential for DOG1 function in vivo. Our study demonstrates that AHG1 and DOG1 constitute an important regulatory system for seed dormancy and germination by integrating multiple environmental signals, in parallel with the PYL/RCAR ABA receptor-mediated regulatory system.

AB - Abscisic acid (ABA) regulates abiotic stress and developmental responses including regulation of seed dormancy to prevent seeds from germinating under unfavorable environmental conditions. ABA HYPERSENSITIVE GERMINATION1 (AHG1) encoding a type 2C protein phosphatase (PP2C) is a central negative regulator of ABA response in germination; however, the molecular function and regulation of AHG1 remain elusive. Here we report that AHG1 interacts with DELAY OF GERMINATION1 (DOG1), which is a pivotal positive regulator in seed dormancy. DOG1 acts upstream of AHG1 and impairs the PP2C activity of AHG1 in vitro. Furthermore, DOG1 has the ability to bind heme. Binding of DOG1 to AHG1 and heme are independent processes, but both are essential for DOG1 function in vivo. Our study demonstrates that AHG1 and DOG1 constitute an important regulatory system for seed dormancy and germination by integrating multiple environmental signals, in parallel with the PYL/RCAR ABA receptor-mediated regulatory system.

UR - http://www.scopus.com/inward/record.url?scp=85048307618&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85048307618&partnerID=8YFLogxK

U2 - 10.1038/s41467-018-04437-9

DO - 10.1038/s41467-018-04437-9

M3 - Article

VL - 9

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 2132

ER -