Control of seed dormancy and germination by DOG1-AHG1 PP2C phosphatase complex via binding to heme

Noriyuki Nishimura, Wataru Tsuchiya, James J. Moresco, Yuki Hayashi, Kouji Satoh, Nahomi Kaiwa, Tomoko Irisa, Toshinori Kinoshita, Julian I. Schroeder, John R. Yates, Takashi Hirayama, Toshimasa Yamazaki

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46 Citations (Scopus)

Abstract

Abscisic acid (ABA) regulates abiotic stress and developmental responses including regulation of seed dormancy to prevent seeds from germinating under unfavorable environmental conditions. ABA HYPERSENSITIVE GERMINATION1 (AHG1) encoding a type 2C protein phosphatase (PP2C) is a central negative regulator of ABA response in germination; however, the molecular function and regulation of AHG1 remain elusive. Here we report that AHG1 interacts with DELAY OF GERMINATION1 (DOG1), which is a pivotal positive regulator in seed dormancy. DOG1 acts upstream of AHG1 and impairs the PP2C activity of AHG1 in vitro. Furthermore, DOG1 has the ability to bind heme. Binding of DOG1 to AHG1 and heme are independent processes, but both are essential for DOG1 function in vivo. Our study demonstrates that AHG1 and DOG1 constitute an important regulatory system for seed dormancy and germination by integrating multiple environmental signals, in parallel with the PYL/RCAR ABA receptor-mediated regulatory system.

Original languageEnglish
Article number2132
JournalNature communications
Volume9
Issue number1
DOIs
Publication statusPublished - Dec 1 2018

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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    Nishimura, N., Tsuchiya, W., Moresco, J. J., Hayashi, Y., Satoh, K., Kaiwa, N., Irisa, T., Kinoshita, T., Schroeder, J. I., Yates, J. R., Hirayama, T., & Yamazaki, T. (2018). Control of seed dormancy and germination by DOG1-AHG1 PP2C phosphatase complex via binding to heme. Nature communications, 9(1), [2132]. https://doi.org/10.1038/s41467-018-04437-9