Contribution of protein p40 to hypovirus-mediated modulation of fungal host phenotype and viral RNA accumulation

Nobuhiro Suzuki, Donald L. Nuss

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The papain-like protease p29, derived from the N-terminal portion of the hypovirus CHV1-EP713-encoded open reading frame (ORF) A polyprotein, p69, was previously shown to contribute to reduced pigmentation and sporulation by the infected host, the chestnut blight fungus Cryphonectria parasitica, while being dispensable for virus replication and attenuation of fungal virulence (hypovirulence). We now report that deletion of the C-terminal portion of p69, which encodes the highly basic protein p40, resulted in replication-competent mutant viruses that were, however, significantly reduced in RNA accumulation. While the Δp40 mutants retained the ability to confer hypovirulence, Δp40-infected fungal strains produced more asexual spores than strains infected with either wild-type CHV1-EP713 or a Δp29 mutant virus. As observed for Δp29-infected colonies, pigment production was significantly increased in Δp40-infected fungal strains relative to that in CHV1-EP713-infected strains. Virus-mediated suppression of laccase production was not affected by p40 deletion. A gain-of-function analysis was employed to map the p40 symptom determinant to the N-terminal domain, encompassing p69 amino acid residues Thr(288) to Arg(312). Evidence that the gain of function was due to the encoded protein rather than the corresponding RNA sequence element was provided by introducing frameshift mutations on either side of the activity determinant domain. Moreover, restoration of symptoms correlated with increased accumulation of viral RNA. These results suggest that p40 indirectly contributes to virus-mediated suppression of fungal pigmentation and conidiation by providing an accessory function in hypovirus RNA amplification. A possible role for p40 in facilitating ORF B expression and the relationship between hypovirus RNA accumulation and symptom expression are discussed.

Original languageEnglish
Pages (from-to)7747-7759
Number of pages13
JournalJournal of Virology
Volume76
Issue number15
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Hypovirus
Viral RNA
RNA
Phenotype
Cryphonectria parasitica
phenotype
viruses
virulence
pigmentation
mutants
open reading frames
Proteins
proteins
Viruses
frameshift mutation
papain
laccase
Pigmentation
virus replication
sporulation

ASJC Scopus subject areas

  • Immunology

Cite this

Contribution of protein p40 to hypovirus-mediated modulation of fungal host phenotype and viral RNA accumulation. / Suzuki, Nobuhiro; Nuss, Donald L.

In: Journal of Virology, Vol. 76, No. 15, 2002, p. 7747-7759.

Research output: Contribution to journalArticle

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