Contrast media increase vascular endothelial permeability by inhibiting nitric-oxide production

Wakako Furuta, Atsushi Yamauchi, Shinya Dohgu, Sinsuke Nakagawa, Toshiaki Sendo, Kazutaka Makino, Ryozo Oishi, Yasufumi Kataoka

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

RATIONALE AND OBJECTIVES. Contrast media induce adverse effects including edema of the face, glottis, or lung. The endothelial function is maintained by nitric oxide (NO). The present study was designed to elucidate the role of NO in mediating endothelium-related adverse effects of contrast media. METHODS. Human microvascular endothelial cells grown on a Transwell membrane were incubated with iohexol or ioxaglate in the absence or presence of NG-monomethyl-L-arginine or sodium nitroprusside. After washing cells, the permeability of sodium fluorescein or Evans blue albumin and the accumulation of NO2- was examined. RESULTS. Contrast media (50-150 mgI/mL) dose-dependently increased the permeability coefficient by 30% to 230% and inhibited the formation of NO2- by 40% to 80%. Sodium nitroprusside and NG-monomethyl-L-arginine produced protective and aggravating effects on contrast media-increased permeability, respectively. CONCLUSIONS. The present study suggested that contrast media increase vascular endothelial permeability by inhibiting NO production, leading to vascular endothelium-related adverse effects of contrast media.

Original languageEnglish
Pages (from-to)13-19
Number of pages7
JournalInvestigative Radiology
Volume37
Issue number1
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Capillary Permeability
Contrast Media
Nitric Oxide
omega-N-Methylarginine
Permeability
Nitroprusside
Ioxaglic Acid
Glottis
Iohexol
Evans Blue
Vascular Endothelium
Fluorescein
Endothelium
Albumins
Edema
Endothelial Cells
Lung
Membranes

Keywords

  • Contrast media
  • Human
  • Microvascular endothelial cells
  • Nitric oxide
  • Vascular permeability

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Contrast media increase vascular endothelial permeability by inhibiting nitric-oxide production. / Furuta, Wakako; Yamauchi, Atsushi; Dohgu, Shinya; Nakagawa, Sinsuke; Sendo, Toshiaki; Makino, Kazutaka; Oishi, Ryozo; Kataoka, Yasufumi.

In: Investigative Radiology, Vol. 37, No. 1, 2002, p. 13-19.

Research output: Contribution to journalArticle

Furuta, W, Yamauchi, A, Dohgu, S, Nakagawa, S, Sendo, T, Makino, K, Oishi, R & Kataoka, Y 2002, 'Contrast media increase vascular endothelial permeability by inhibiting nitric-oxide production', Investigative Radiology, vol. 37, no. 1, pp. 13-19. https://doi.org/10.1097/00004424-200201000-00004
Furuta, Wakako ; Yamauchi, Atsushi ; Dohgu, Shinya ; Nakagawa, Sinsuke ; Sendo, Toshiaki ; Makino, Kazutaka ; Oishi, Ryozo ; Kataoka, Yasufumi. / Contrast media increase vascular endothelial permeability by inhibiting nitric-oxide production. In: Investigative Radiology. 2002 ; Vol. 37, No. 1. pp. 13-19.
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AU - Dohgu, Shinya

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AU - Sendo, Toshiaki

AU - Makino, Kazutaka

AU - Oishi, Ryozo

AU - Kataoka, Yasufumi

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N2 - RATIONALE AND OBJECTIVES. Contrast media induce adverse effects including edema of the face, glottis, or lung. The endothelial function is maintained by nitric oxide (NO). The present study was designed to elucidate the role of NO in mediating endothelium-related adverse effects of contrast media. METHODS. Human microvascular endothelial cells grown on a Transwell membrane were incubated with iohexol or ioxaglate in the absence or presence of NG-monomethyl-L-arginine or sodium nitroprusside. After washing cells, the permeability of sodium fluorescein or Evans blue albumin and the accumulation of NO2- was examined. RESULTS. Contrast media (50-150 mgI/mL) dose-dependently increased the permeability coefficient by 30% to 230% and inhibited the formation of NO2- by 40% to 80%. Sodium nitroprusside and NG-monomethyl-L-arginine produced protective and aggravating effects on contrast media-increased permeability, respectively. CONCLUSIONS. The present study suggested that contrast media increase vascular endothelial permeability by inhibiting NO production, leading to vascular endothelium-related adverse effects of contrast media.

AB - RATIONALE AND OBJECTIVES. Contrast media induce adverse effects including edema of the face, glottis, or lung. The endothelial function is maintained by nitric oxide (NO). The present study was designed to elucidate the role of NO in mediating endothelium-related adverse effects of contrast media. METHODS. Human microvascular endothelial cells grown on a Transwell membrane were incubated with iohexol or ioxaglate in the absence or presence of NG-monomethyl-L-arginine or sodium nitroprusside. After washing cells, the permeability of sodium fluorescein or Evans blue albumin and the accumulation of NO2- was examined. RESULTS. Contrast media (50-150 mgI/mL) dose-dependently increased the permeability coefficient by 30% to 230% and inhibited the formation of NO2- by 40% to 80%. Sodium nitroprusside and NG-monomethyl-L-arginine produced protective and aggravating effects on contrast media-increased permeability, respectively. CONCLUSIONS. The present study suggested that contrast media increase vascular endothelial permeability by inhibiting NO production, leading to vascular endothelium-related adverse effects of contrast media.

KW - Contrast media

KW - Human

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KW - Nitric oxide

KW - Vascular permeability

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