Consumption of indigestible saccharides and administration of Bifidobacterium pseudolongum reduce mucosal serotonin in murine colonic mucosa

Misa Tatsuoka, Yosuke Osaki, Fumina Ohsaka, Takeshi Tsuruta, Yoshihiro Kadota, Takumi Tochio, Shingo Hino, Tatsuya Morita, Kei Sonoyama

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Short-chain fatty acids (SCFAs) increase serotonin (5-hydroxytryptamine, 5-HT) synthesis and content in the colon in vitro and ex vivo, but little is known in vivo. We tested whether dietary indigestible saccharides, utilized as a substrate to produce SCFAs by gut microbiota, would increase colonic 5-HT content in mice. Male C57BL/6J mice were fed a purified diet and water supplemented with 4% (w/v) 1-kestose (KES) for 2 weeks. Colonic 5-HT content and enterochromaffin (EC) cell numbers were lower in mice supplemented with KES than those without supplementation, while monoamine oxidase A activity and mRNA levels of Tph1, Chga, Slc6a4 and Maoa genes in the colonic mucosa, serum 5-HT concentration and total 5-HT content in the colonic contents did not differ between groups. Cecal acetate concentration and Bifidobacterium pseudolongum population were higher in KES-supplemented mice. Similar trends were observed in mice supplemented with other indigestible saccharides, i.e., fructooligosaccharides, inulin and raffinose. Intragastric administration of live B. pseudolongum (108 colony-forming units/day) for 2 weeks reduced colonic 5-HT content and EC cell numbers. These results suggest that changes in synthesis, reuptake, catabolism and overflow of 5-HT in the colonic mucosa are not involved in the reduction of colonic 5-HT content by dietary indigestible saccharides in mice. We propose that gut microbes including B. pseudolongum could contribute to the reduction of 5-HT content in the colonic mucosa via diminishing EC cells.

Original languageEnglish
JournalBritish Journal of Nutrition
DOIs
Publication statusAccepted/In press - 2021

Keywords

  • 1-kestose
  • DSS-induced colitis
  • Keywords:
  • bifidobacteria
  • gut microbiota
  • serotonin
  • short-chain fatty acid

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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