Construction of plants resistant to TYLCV by using artificial zinc-finger proteins.

Yoshihiro Koshino-Kimura, Kosuke Takenaka, Fumiya Domoto, Masayoshi Ohashi, Toshihide Miyazaki, Yasuhiro Aoyama, Takashi Sera

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Previously, we have demonstrated that plant DNA virus replication could be inhibited in Arabidopsis thaliana by using an artificial zinc-finger protein (AZP) and created AZP-based transgenic A. thaliana resistant to DNA virus infection. Here we apply the AZP technology to tomato yellow leaf curl virus (TYLCV) causing serious damage to an important agricultural crop, tomato. An AZP was designed to block binding of the TYLCV replication protein (Rep) to the replication origin. The designed AZP had much higher affinities towards the replication origin than did the Rep, and efficiently blocked Rep binding in vitro. The AZP gene was then introduced into a plant genome with the help of Agrobacterium tumefaciens to generate the transgenic plants. The current status of the construction of the AZP-expressing transgenic plants will be reported.

Original languageEnglish
Pages (from-to)281-282
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number53
Publication statusPublished - 2009
Externally publishedYes

Fingerprint

Zinc Fingers
Lycopersicon esculentum
Viruses
Proteins
Replication Origin
Genetically Modified Plants
Virus Replication
Arabidopsis
DNA Virus Infections
Agricultural Crops
Plant DNA
Plant Genome
Plant Viruses
Agrobacterium tumefaciens
DNA Viruses
DNA Replication
Protein Binding
Technology

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Koshino-Kimura, Y., Takenaka, K., Domoto, F., Ohashi, M., Miyazaki, T., Aoyama, Y., & Sera, T. (2009). Construction of plants resistant to TYLCV by using artificial zinc-finger proteins. Nucleic acids symposium series (2004), (53), 281-282.

Construction of plants resistant to TYLCV by using artificial zinc-finger proteins. / Koshino-Kimura, Yoshihiro; Takenaka, Kosuke; Domoto, Fumiya; Ohashi, Masayoshi; Miyazaki, Toshihide; Aoyama, Yasuhiro; Sera, Takashi.

In: Nucleic acids symposium series (2004), No. 53, 2009, p. 281-282.

Research output: Contribution to journalArticle

Koshino-Kimura, Y, Takenaka, K, Domoto, F, Ohashi, M, Miyazaki, T, Aoyama, Y & Sera, T 2009, 'Construction of plants resistant to TYLCV by using artificial zinc-finger proteins.', Nucleic acids symposium series (2004), no. 53, pp. 281-282.
Koshino-Kimura Y, Takenaka K, Domoto F, Ohashi M, Miyazaki T, Aoyama Y et al. Construction of plants resistant to TYLCV by using artificial zinc-finger proteins. Nucleic acids symposium series (2004). 2009;(53):281-282.
Koshino-Kimura, Yoshihiro ; Takenaka, Kosuke ; Domoto, Fumiya ; Ohashi, Masayoshi ; Miyazaki, Toshihide ; Aoyama, Yasuhiro ; Sera, Takashi. / Construction of plants resistant to TYLCV by using artificial zinc-finger proteins. In: Nucleic acids symposium series (2004). 2009 ; No. 53. pp. 281-282.
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