Construction of a series of mutants lacking all of the four major mex operons for multidrug efflux pumps or possessing each one of the operons from Pseudomonas aeruginosa PAO1

MexCD-OprJ is an inducible pump

Yuji Morita, Yukiko Komori, Takehiko Mima, Teruo Kuroda, Tohru Mizushima, Tomofusa Tsuchiya

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

We constructed a series of deletion mutants lacking all of the four major mex operons for Mex multidrug efflux pumps or possessing each one of the operons from Pseudomonas aeruginosa PAO1. The drug specificity of MexAB-OprM, MexXY-OprM and MexCD-OprJ was investigated. Surprisingly, we found that the MexCD-OprJ was an inducible pump, inducers of which were tetraphenylphosphonium chloride, ethidium bromide, rhodamine 6G and acriflavine. Fluoroquinolones, chloramphenicol, erythromycin and tetracycline were not inducers although they were substrates of MexCD-OprJ.

Original languageEnglish
Pages (from-to)139-143
Number of pages5
JournalFEMS Microbiology Letters
Volume202
Issue number1
DOIs
Publication statusPublished - Aug 7 2001

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Operon
Pseudomonas aeruginosa
Acriflavine
Ethidium
Fluoroquinolones
Chloramphenicol
Erythromycin
Tetracycline
Pharmaceutical Preparations
rhodamine 6G
tetraphenylphosphonium

Keywords

  • A series of deletion mutants
  • Drug specificity
  • Mex multidrug efflux pump
  • MexCD-OprM
  • Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Construction of a series of mutants lacking all of the four major mex operons for multidrug efflux pumps or possessing each one of the operons from Pseudomonas aeruginosa PAO1 : MexCD-OprJ is an inducible pump. / Morita, Yuji; Komori, Yukiko; Mima, Takehiko; Kuroda, Teruo; Mizushima, Tohru; Tsuchiya, Tomofusa.

In: FEMS Microbiology Letters, Vol. 202, No. 1, 07.08.2001, p. 139-143.

Research output: Contribution to journalArticle

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