Construction and characterization of the PGN_0296 mutant of Porphyromonas gingivalis

Abu Saleh Muhammad Shahriar, Shintaro Ono, Masaaki Nakayama, Naoko Ohara, Naoya Ohara

Research output: Contribution to journalArticlepeer-review

Abstract

The periodontal pathogen Porphyromonas gingivalis produces gingipains (Kgp, RgpA, and RgpB), cysteine proteases involved in the organism's virulence, and pigmentation. We previously showed that deletion of the PGN_0297 and PGN_0300 genes reduced the proteolytic activity of gingipains. The role of the PGN_0296 gene, consisting of an operon with the PGN_0297 and PGN_0300 genes, is unclear. Herein, we examined the effect of PGN_0296 gene deletion on the proteolytic activity. Although the proteolytic activity of the gingipains did not decrease in the culture supernatant of a PGN_0296 gene deletion mutant (ΔPGN_0296), the growth was delayed.

Original languageEnglish
Journaljournal of oral biosciences
DOIs
Publication statusAccepted/In press - 2020

Keywords

  • Bacterial secretion system
  • Gingipain
  • Periodontal diseases
  • Porphyromonas gingivalis

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Dentistry(all)

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