Construction and Characterization of a PGN_0297 Mutant of Porphyromonas gingivalis: Evidence of the Contribution of PGN_0297 to Gingipain Activity

Shintaro Ono, Masaaki Nakayama, Masato Tachibana, Abu Saleh Muhammad Shahriar, Wang Heling, Shogo Takashiba, Naoya Oohara

Research output: Contribution to journalArticle

Abstract

The periodontal pathogen Porphyromonas gingivalis shows colonial pigmentation on blood agar and produces gingipains (Kgp, RgpA, and RgpB), cysteine proteases involved in an organism's virulence and pigmentation. We showed previously that deletion of the PGN_0300 gene abolished the pigmentation activity and reduced the proteolytic activity of gingipains. The role of the PGN_0297 gene, which consists of an operon with the PGN_0300 gene, is unclear. Herein we examined the effect of PGN_0297 gene deletion on the pigmentation and proteolytic activities and transcriptional levels of gingipains. A PGN_0297 gene deletion mutant (ΔPGN_0297) did not exhibit the pigmentation. The proteolytic activity of the gingipains was decreased in the culture supernatant and on the cell surface of ΔPGN_0297. The mutant ΔPGN_0297 failed to attenuate Akt phosphorylation at Thr308 and Ser473, but both phosphorylations were attenuated in the wild-type and its complementation strain. The deletion of PGN_0297 gene did not substantially affect the transcriptional levels of the gingipain genes kgp, rgpA, and rgpB. Taken together, these results indicate that PGN_0297 is closely involved in the secretion and maturation of gingipains.

Original languageEnglish
Pages (from-to)315-323
Number of pages9
JournalActa medica Okayama
Volume73
Issue number4
DOIs
Publication statusPublished - Aug 1 2019

Fingerprint

Porphyromonas gingivalis
Pigmentation
Genes
Gene Deletion
Phosphorylation
Cysteine Proteases
Operon
Pathogens
Agar
Virulence
Porphyromonas gingivalis argingipain
Blood

Keywords

  • C-terminal domain
  • gingipain
  • periodontitis
  • Porphyromonas gingivalis
  • secretion system

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Construction and Characterization of a PGN_0297 Mutant of Porphyromonas gingivalis : Evidence of the Contribution of PGN_0297 to Gingipain Activity. / Ono, Shintaro; Nakayama, Masaaki; Tachibana, Masato; Shahriar, Abu Saleh Muhammad; Heling, Wang; Takashiba, Shogo; Oohara, Naoya.

In: Acta medica Okayama, Vol. 73, No. 4, 01.08.2019, p. 315-323.

Research output: Contribution to journalArticle

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