Abstract
The periodontal pathogen Porphyromonas gingivalis shows colonial pigmentation on blood agar and produces gingipains (Kgp, RgpA, and RgpB), cysteine proteases involved in an organism's virulence and pigmentation. We showed previously that deletion of the PGN_0300 gene abolished the pigmentation activity and reduced the proteolytic activity of gingipains. The role of the PGN_0297 gene, which consists of an operon with the PGN_0300 gene, is unclear. Herein we examined the effect of PGN_0297 gene deletion on the pigmentation and proteolytic activities and transcriptional levels of gingipains. A PGN_0297 gene deletion mutant (ΔPGN_0297) did not exhibit the pigmentation. The proteolytic activity of the gingipains was decreased in the culture supernatant and on the cell surface of ΔPGN_0297. The mutant ΔPGN_0297 failed to attenuate Akt phosphorylation at Thr308 and Ser473, but both phosphorylations were attenuated in the wild-type and its complementation strain. The deletion of PGN_0297 gene did not substantially affect the transcriptional levels of the gingipain genes kgp, rgpA, and rgpB. Taken together, these results indicate that PGN_0297 is closely involved in the secretion and maturation of gingipains.
| Original language | English |
|---|---|
| Pages (from-to) | 315-323 |
| Number of pages | 9 |
| Journal | Acta medica Okayama |
| Volume | 73 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Aug 1 2019 |
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Keywords
- C-terminal domain
- gingipain
- periodontitis
- Porphyromonas gingivalis
- secretion system
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Construction and Characterization of a PGN_0297 Mutant of Porphyromonas gingivalis : Evidence of the Contribution of PGN_0297 to Gingipain Activity. / Ono, Shintaro; Nakayama, Masaaki; Tachibana, Masato; Shahriar, Abu Saleh Muhammad; Heling, Wang; Takashiba, Shogo; Oohara, Naoya.
In: Acta medica Okayama, Vol. 73, No. 4, 01.08.2019, p. 315-323.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Construction and Characterization of a PGN_0297 Mutant of Porphyromonas gingivalis
T2 - Evidence of the Contribution of PGN_0297 to Gingipain Activity
AU - Ono, Shintaro
AU - Nakayama, Masaaki
AU - Tachibana, Masato
AU - Shahriar, Abu Saleh Muhammad
AU - Heling, Wang
AU - Takashiba, Shogo
AU - Oohara, Naoya
PY - 2019/8/1
Y1 - 2019/8/1
N2 - The periodontal pathogen Porphyromonas gingivalis shows colonial pigmentation on blood agar and produces gingipains (Kgp, RgpA, and RgpB), cysteine proteases involved in an organism's virulence and pigmentation. We showed previously that deletion of the PGN_0300 gene abolished the pigmentation activity and reduced the proteolytic activity of gingipains. The role of the PGN_0297 gene, which consists of an operon with the PGN_0300 gene, is unclear. Herein we examined the effect of PGN_0297 gene deletion on the pigmentation and proteolytic activities and transcriptional levels of gingipains. A PGN_0297 gene deletion mutant (ΔPGN_0297) did not exhibit the pigmentation. The proteolytic activity of the gingipains was decreased in the culture supernatant and on the cell surface of ΔPGN_0297. The mutant ΔPGN_0297 failed to attenuate Akt phosphorylation at Thr308 and Ser473, but both phosphorylations were attenuated in the wild-type and its complementation strain. The deletion of PGN_0297 gene did not substantially affect the transcriptional levels of the gingipain genes kgp, rgpA, and rgpB. Taken together, these results indicate that PGN_0297 is closely involved in the secretion and maturation of gingipains.
AB - The periodontal pathogen Porphyromonas gingivalis shows colonial pigmentation on blood agar and produces gingipains (Kgp, RgpA, and RgpB), cysteine proteases involved in an organism's virulence and pigmentation. We showed previously that deletion of the PGN_0300 gene abolished the pigmentation activity and reduced the proteolytic activity of gingipains. The role of the PGN_0297 gene, which consists of an operon with the PGN_0300 gene, is unclear. Herein we examined the effect of PGN_0297 gene deletion on the pigmentation and proteolytic activities and transcriptional levels of gingipains. A PGN_0297 gene deletion mutant (ΔPGN_0297) did not exhibit the pigmentation. The proteolytic activity of the gingipains was decreased in the culture supernatant and on the cell surface of ΔPGN_0297. The mutant ΔPGN_0297 failed to attenuate Akt phosphorylation at Thr308 and Ser473, but both phosphorylations were attenuated in the wild-type and its complementation strain. The deletion of PGN_0297 gene did not substantially affect the transcriptional levels of the gingipain genes kgp, rgpA, and rgpB. Taken together, these results indicate that PGN_0297 is closely involved in the secretion and maturation of gingipains.
KW - C-terminal domain
KW - gingipain
KW - periodontitis
KW - Porphyromonas gingivalis
KW - secretion system
UR - http://www.scopus.com/inward/record.url?scp=85071446911&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85071446911&partnerID=8YFLogxK
U2 - 10.18926/AMO/56933
DO - 10.18926/AMO/56933
M3 - Article
C2 - 31439954
AN - SCOPUS:85071446911
VL - 73
SP - 315
EP - 323
JO - Acta Medica Okayama
JF - Acta Medica Okayama
SN - 0386-300X
IS - 4
ER -