Connective tissue growth factor is overexpressed in muscles of human muscular dystrophy

Guilian Sun, Kazuhiro Haginoya, Yanling Wu, Yoko Chiba, Tohru Nakanishi, Akira Onuma, Yuko Sato, Masaharu Takigawa, Kazuie Iinuma, Shigeru Tsuchiya

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

The detailed process of how dystrophic muscles are replaced by fibrotic tissues is unknown. In the present study, the immunolocalization and mRNA expression of connective tissue growth factor (CTGF) in muscles from normal and dystrophic human muscles were examined with the goal of elucidating the pathophysiological function of CTGF in muscular dystrophy. Biopsies of frozen muscle from patients with Duchenne muscular dystrophy (DMD), Becker muscular dystrophy, congenital muscular dystrophy, spinal muscular atrophy, congenital myopathy were analyzed using anti-CTGF polyclonal antibody. Reverse transcription-polymerase chain reaction (RT-PCR) was also performed to evaluate the expression of CTGF mRNA in dystrophic muscles. In normal muscle, neuromuscular junctions and vessels were CTGF-immunopositive, which suggests a physiological role for CTGF in these sites. In dystrophic muscle, CTGF immunoreactivity was localized to muscle fiber basal lamina, regenerating fibers, and the interstitium. Triple immunolabeling revealed that activated fibroblasts were immunopositive for CTGF and transforming growth factor-beta1 (TGF-beta1). RT-PCR analysis revealed increased levels of CTGF mRNA in the muscles of DMD patients. Co-localization of TGF-beta1 and CTGF in activated fibroblasts suggests that CTGF expression is regulated by TGF-beta1 through a paracrine/autocrine mechanism. In conclusion, TGF-beta1-CTGF pathway may play a role in the fibrosis that is commonly observed in muscular dystrophy.

Original languageEnglish
Pages (from-to)48-56
Number of pages9
JournalJournal of the neurological sciences
Volume267
Issue number1-2
DOIs
Publication statusPublished - Apr 15 2008

Keywords

  • Connective tissue growth factor
  • Fibrosis
  • Immunohistochemistry
  • Muscular dystrophy
  • RT-PCR
  • Transforming growth factor

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

Fingerprint Dive into the research topics of 'Connective tissue growth factor is overexpressed in muscles of human muscular dystrophy'. Together they form a unique fingerprint.

  • Cite this

    Sun, G., Haginoya, K., Wu, Y., Chiba, Y., Nakanishi, T., Onuma, A., Sato, Y., Takigawa, M., Iinuma, K., & Tsuchiya, S. (2008). Connective tissue growth factor is overexpressed in muscles of human muscular dystrophy. Journal of the neurological sciences, 267(1-2), 48-56. https://doi.org/10.1016/j.jns.2007.09.043