Connective tissue growth factor induces the proliferation, migration, and tube formation of vascular endothelial cells in vitro, and angiogenesis in vivo

Tsuyoshi Shimo, Tohru Nakanishi, Takashi Nishida, Masahiro Asano, Manabu Kanyama, Takuo Kuboki, Takuya Tamatani, Katsunari Tezuka, Motohide Takemura, Tomohiro Matsumura, Masaharu Takigawa

Research output: Contribution to journalArticle

331 Citations (Scopus)

Abstract

Connective tissue growth factor (CTGF) is a novel cysteine-rich, secreted protein. Recently, we found that inhibition of the endogenous expression of CTGF by its antisense oligonucleotide and antisense RNA suppresses the proliferation and migration of vascular endothelial cells. In the present study, the following observations demonstrated the angiogenic function of CTGF in vitro and in vivo: (i) purified recombinant CTGF (rCTGF) promoted the adhesion, proliferation and migration of vascular endothelial cells in a dose-dependent manner under serum-free conditions, and these effects were inhibited by anti-CTGF antibodies; (ii) rCTGF markedly induced the tube formation of vascular endothelial cells, and this effect was stronger than that of basic fibroblast growth factor or vascular endothelial growth factor; (iii) application of rCTGF to the chicken chorioallantoic membrane resulted in a gross angiogenic response, and this effect was also inhibited by anti-CTGF antibodies. (iv) rCTGF injected with collagen gel into the backs of mice induced strong angiogenesis in vivo. These findings indicate that CTGF is a novel, potent angiogenesis factor which functions in multi-stages in this process.

Original languageEnglish
Pages (from-to)137-145
Number of pages9
JournalJournal of Biochemistry
Volume126
Issue number1
Publication statusPublished - 1999

Fingerprint

Connective Tissue Growth Factor
Endothelial cells
Endothelial Cells
Chorioallantoic Membrane
Antisense RNA
Antibodies
Antisense Oligonucleotides
Angiogenesis Inducing Agents
Fibroblast Growth Factor 2
In Vitro Techniques
Vascular Endothelial Growth Factor A
Cysteine
Chickens
Collagen
Adhesion
Gels
Membranes
Serum

Keywords

  • Angiogenesis
  • Connective tissue growth factor (CTGF)
  • Endothelial cells
  • Hypertrophic chondrocytes
  • Neovascularization

ASJC Scopus subject areas

  • Biochemistry

Cite this

Connective tissue growth factor induces the proliferation, migration, and tube formation of vascular endothelial cells in vitro, and angiogenesis in vivo. / Shimo, Tsuyoshi; Nakanishi, Tohru; Nishida, Takashi; Asano, Masahiro; Kanyama, Manabu; Kuboki, Takuo; Tamatani, Takuya; Tezuka, Katsunari; Takemura, Motohide; Matsumura, Tomohiro; Takigawa, Masaharu.

In: Journal of Biochemistry, Vol. 126, No. 1, 1999, p. 137-145.

Research output: Contribution to journalArticle

Shimo, Tsuyoshi ; Nakanishi, Tohru ; Nishida, Takashi ; Asano, Masahiro ; Kanyama, Manabu ; Kuboki, Takuo ; Tamatani, Takuya ; Tezuka, Katsunari ; Takemura, Motohide ; Matsumura, Tomohiro ; Takigawa, Masaharu. / Connective tissue growth factor induces the proliferation, migration, and tube formation of vascular endothelial cells in vitro, and angiogenesis in vivo. In: Journal of Biochemistry. 1999 ; Vol. 126, No. 1. pp. 137-145.
@article{794b3eb54a3b450a85caa1a569c5cd86,
title = "Connective tissue growth factor induces the proliferation, migration, and tube formation of vascular endothelial cells in vitro, and angiogenesis in vivo",
abstract = "Connective tissue growth factor (CTGF) is a novel cysteine-rich, secreted protein. Recently, we found that inhibition of the endogenous expression of CTGF by its antisense oligonucleotide and antisense RNA suppresses the proliferation and migration of vascular endothelial cells. In the present study, the following observations demonstrated the angiogenic function of CTGF in vitro and in vivo: (i) purified recombinant CTGF (rCTGF) promoted the adhesion, proliferation and migration of vascular endothelial cells in a dose-dependent manner under serum-free conditions, and these effects were inhibited by anti-CTGF antibodies; (ii) rCTGF markedly induced the tube formation of vascular endothelial cells, and this effect was stronger than that of basic fibroblast growth factor or vascular endothelial growth factor; (iii) application of rCTGF to the chicken chorioallantoic membrane resulted in a gross angiogenic response, and this effect was also inhibited by anti-CTGF antibodies. (iv) rCTGF injected with collagen gel into the backs of mice induced strong angiogenesis in vivo. These findings indicate that CTGF is a novel, potent angiogenesis factor which functions in multi-stages in this process.",
keywords = "Angiogenesis, Connective tissue growth factor (CTGF), Endothelial cells, Hypertrophic chondrocytes, Neovascularization",
author = "Tsuyoshi Shimo and Tohru Nakanishi and Takashi Nishida and Masahiro Asano and Manabu Kanyama and Takuo Kuboki and Takuya Tamatani and Katsunari Tezuka and Motohide Takemura and Tomohiro Matsumura and Masaharu Takigawa",
year = "1999",
language = "English",
volume = "126",
pages = "137--145",
journal = "Journal of Biochemistry",
issn = "0021-924X",
publisher = "Oxford University Press",
number = "1",

}

TY - JOUR

T1 - Connective tissue growth factor induces the proliferation, migration, and tube formation of vascular endothelial cells in vitro, and angiogenesis in vivo

AU - Shimo, Tsuyoshi

AU - Nakanishi, Tohru

AU - Nishida, Takashi

AU - Asano, Masahiro

AU - Kanyama, Manabu

AU - Kuboki, Takuo

AU - Tamatani, Takuya

AU - Tezuka, Katsunari

AU - Takemura, Motohide

AU - Matsumura, Tomohiro

AU - Takigawa, Masaharu

PY - 1999

Y1 - 1999

N2 - Connective tissue growth factor (CTGF) is a novel cysteine-rich, secreted protein. Recently, we found that inhibition of the endogenous expression of CTGF by its antisense oligonucleotide and antisense RNA suppresses the proliferation and migration of vascular endothelial cells. In the present study, the following observations demonstrated the angiogenic function of CTGF in vitro and in vivo: (i) purified recombinant CTGF (rCTGF) promoted the adhesion, proliferation and migration of vascular endothelial cells in a dose-dependent manner under serum-free conditions, and these effects were inhibited by anti-CTGF antibodies; (ii) rCTGF markedly induced the tube formation of vascular endothelial cells, and this effect was stronger than that of basic fibroblast growth factor or vascular endothelial growth factor; (iii) application of rCTGF to the chicken chorioallantoic membrane resulted in a gross angiogenic response, and this effect was also inhibited by anti-CTGF antibodies. (iv) rCTGF injected with collagen gel into the backs of mice induced strong angiogenesis in vivo. These findings indicate that CTGF is a novel, potent angiogenesis factor which functions in multi-stages in this process.

AB - Connective tissue growth factor (CTGF) is a novel cysteine-rich, secreted protein. Recently, we found that inhibition of the endogenous expression of CTGF by its antisense oligonucleotide and antisense RNA suppresses the proliferation and migration of vascular endothelial cells. In the present study, the following observations demonstrated the angiogenic function of CTGF in vitro and in vivo: (i) purified recombinant CTGF (rCTGF) promoted the adhesion, proliferation and migration of vascular endothelial cells in a dose-dependent manner under serum-free conditions, and these effects were inhibited by anti-CTGF antibodies; (ii) rCTGF markedly induced the tube formation of vascular endothelial cells, and this effect was stronger than that of basic fibroblast growth factor or vascular endothelial growth factor; (iii) application of rCTGF to the chicken chorioallantoic membrane resulted in a gross angiogenic response, and this effect was also inhibited by anti-CTGF antibodies. (iv) rCTGF injected with collagen gel into the backs of mice induced strong angiogenesis in vivo. These findings indicate that CTGF is a novel, potent angiogenesis factor which functions in multi-stages in this process.

KW - Angiogenesis

KW - Connective tissue growth factor (CTGF)

KW - Endothelial cells

KW - Hypertrophic chondrocytes

KW - Neovascularization

UR - http://www.scopus.com/inward/record.url?scp=0032817965&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032817965&partnerID=8YFLogxK

M3 - Article

VL - 126

SP - 137

EP - 145

JO - Journal of Biochemistry

JF - Journal of Biochemistry

SN - 0021-924X

IS - 1

ER -