TY - JOUR
T1 - Connective tissue growth factor expressed in rat alveolar bone regeneration sites after tooth extraction
AU - Kanyama, Manabu
AU - Kuboki, Takuo
AU - Akiyama, Kentaro
AU - Nawachi, Kumiko
AU - Miyauchi, Fabiora M.
AU - Yatani, Hirofumi
AU - Kubota, Satoshi
AU - Nakanishi, Tohru
AU - Takigawa, Masaharu
N1 - Funding Information:
This study was supported in part by Grant-In-Aids from the Ministry of Education, Culture, Sports, Science and Technology of Japan (No. 12470419, 15592050, 15390592, 12470419, 13307053, 15109010, 15592050, 15390592).
PY - 2003/10/1
Y1 - 2003/10/1
N2 - Objective: To understand bone regeneration process after tooth extraction could be a clue to develop a new strategy for alveolar bone reconstruction. Recently, accumulated evidences support that connective tissue growth factor (CTGF) is implicated in tissue repair of many tissues. In this study, we investigated the spatial and temporal expression of CTGF in the rat tooth extraction sockets. Design: Five weeks old wild type male rats (weighing 120 g) were used for this experiment. Expression of CTGF was determined by immunohistochemistry and in situ hybridization in the rat upper molar tooth extraction sockets at 2, 4, 7, 10 and 14 days after tooth extraction. Results: CTGF was expressed strongly in the endothelial cells migrating into the granulation tissue at the bottom of the sockets during 4 days after tooth extraction. During the reparative process, no apparent chondrocyte-like cell appeared in the sockets, while osteoblast-like cells proliferated in the sockets with low CTGF expression at 7, 10, 14 days after extraction. As expected, no staining was observed with the preimmune rabbit IgG and CTGF sense probe. CTGF may play an important role in angiogenesis and granulation tissue formation specifically at early healing stage after tooth extraction to initiate alveolar bone repair. Conclusion: CTGF was expressed at early healing stage of the rat tooth extraction wound.
AB - Objective: To understand bone regeneration process after tooth extraction could be a clue to develop a new strategy for alveolar bone reconstruction. Recently, accumulated evidences support that connective tissue growth factor (CTGF) is implicated in tissue repair of many tissues. In this study, we investigated the spatial and temporal expression of CTGF in the rat tooth extraction sockets. Design: Five weeks old wild type male rats (weighing 120 g) were used for this experiment. Expression of CTGF was determined by immunohistochemistry and in situ hybridization in the rat upper molar tooth extraction sockets at 2, 4, 7, 10 and 14 days after tooth extraction. Results: CTGF was expressed strongly in the endothelial cells migrating into the granulation tissue at the bottom of the sockets during 4 days after tooth extraction. During the reparative process, no apparent chondrocyte-like cell appeared in the sockets, while osteoblast-like cells proliferated in the sockets with low CTGF expression at 7, 10, 14 days after extraction. As expected, no staining was observed with the preimmune rabbit IgG and CTGF sense probe. CTGF may play an important role in angiogenesis and granulation tissue formation specifically at early healing stage after tooth extraction to initiate alveolar bone repair. Conclusion: CTGF was expressed at early healing stage of the rat tooth extraction wound.
KW - Angiogenesis
KW - CTGF
KW - Membranous bone
KW - Tooth extraction sockets
KW - Wound healing
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U2 - 10.1016/S0003-9969(03)00153-5
DO - 10.1016/S0003-9969(03)00153-5
M3 - Article
C2 - 12971950
AN - SCOPUS:0141786808
SN - 0003-9969
VL - 48
SP - 723
EP - 730
JO - Archives of Oral Biology
JF - Archives of Oral Biology
IS - 10
ER -