Connective tissue growth factor as a major angiogenic agent that is induced by hypoxia in a human breast cancer cell line

Tsuyoshi Shimo, Satoshi Kubota, Seiji Kondo, Tohru Nakanishi, Akira Sasaki, Hiroshi Mese, Tomohiro Matsumura, Masaharu Takigawa

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75 Citations (Scopus)


Connective tissue growth factor (CTGF) is known to be a potent angiogenic factor. Here, we present the evidence that the hypoxic induction of angiogenesis by human breast cancer cells (MDA-231) can be ascribed at least in part to CTGF. Our results indicate that (i) CTGF is abundantly present in MDA-231 cells in vitro and in vivo, (ii) its secretion is up-regulated by hypoxia, and (iii) its gene expression is enhanced in MDA-231 cells cultured under hypoxic conditions. These data suggest CTGF may stimulate angiogenesis by paracrine mechanisms, thereby contributing to the invasion of breast cancer cells. This is the first evidence that human cancer cells differentially express CTGF protein and mRNA under the control of hypoxic conditions.

Original languageEnglish
Pages (from-to)57-64
Number of pages8
JournalCancer Letters
Issue number1
Publication statusPublished - Dec 10 2001



  • Angiogenesis
  • Breast cancer
  • Connective tissue growth factor
  • Hypoxia

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

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