Abstract
Monoclonal antibodies were purified from the culture medium of hybridoma from systemic lupus erythematosus (SLE)-prone MRL/Mp-Ipr-Ipr mice with affinity chromatography. Binding activity of the antibodies to double-stranded DNA from salmon milt was actually shown in enzyme-linked immunosorbent assay (ELISA). Circular dichroism (CD) spectrum of DNA solution (150 mM NaCl, 20 mM Na2HPO4, pH 7.4) which showed B-type conformation, changed significantly by addition of the anti-DNA antibodies. These results indicate that conformational change of DNA occurred by binding of anti-DNA antibodies, forming DNA-anti-DNA antibody immune complex.
| Original language | English |
|---|---|
| Pages (from-to) | 145-146 |
| Number of pages | 2 |
| Journal | Nucleic acids symposium series |
| Issue number | 37 |
| Publication status | Published - 1997 |
| Externally published | Yes |
ASJC Scopus subject areas
- Medicine(all)