Conformational change of DNA on the formation of DNA-anti-DNA antibody immune complex.

H. Kitamura, K. Shindo, M. Sasabe, Eiji Matsuura, N. Sakairi, N. Nishi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Monoclonal antibodies were purified from the culture medium of hybridoma from systemic lupus erythematosus (SLE)-prone MRL/Mp-Ipr-Ipr mice with affinity chromatography. Binding activity of the antibodies to double-stranded DNA from salmon milt was actually shown in enzyme-linked immunosorbent assay (ELISA). Circular dichroism (CD) spectrum of DNA solution (150 mM NaCl, 20 mM Na2HPO4, pH 7.4) which showed B-type conformation, changed significantly by addition of the anti-DNA antibodies. These results indicate that conformational change of DNA occurred by binding of anti-DNA antibodies, forming DNA-anti-DNA antibody immune complex.

Original languageEnglish
Pages (from-to)145-146
Number of pages2
JournalNucleic acids symposium series
Issue number37
Publication statusPublished - 1997
Externally publishedYes

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Antinuclear Antibodies
Antigen-Antibody Complex
DNA
Salmon
Hybridomas
Circular Dichroism
Affinity Chromatography
Systemic Lupus Erythematosus
Culture Media
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Antibodies

Cite this

Kitamura, H., Shindo, K., Sasabe, M., Matsuura, E., Sakairi, N., & Nishi, N. (1997). Conformational change of DNA on the formation of DNA-anti-DNA antibody immune complex. Nucleic acids symposium series, (37), 145-146.

Conformational change of DNA on the formation of DNA-anti-DNA antibody immune complex. / Kitamura, H.; Shindo, K.; Sasabe, M.; Matsuura, Eiji; Sakairi, N.; Nishi, N.

In: Nucleic acids symposium series, No. 37, 1997, p. 145-146.

Research output: Contribution to journalArticle

Kitamura, H, Shindo, K, Sasabe, M, Matsuura, E, Sakairi, N & Nishi, N 1997, 'Conformational change of DNA on the formation of DNA-anti-DNA antibody immune complex.', Nucleic acids symposium series, no. 37, pp. 145-146.
Kitamura, H. ; Shindo, K. ; Sasabe, M. ; Matsuura, Eiji ; Sakairi, N. ; Nishi, N. / Conformational change of DNA on the formation of DNA-anti-DNA antibody immune complex. In: Nucleic acids symposium series. 1997 ; No. 37. pp. 145-146.
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