Conformational change of DNA on the formation of DNA-anti-DNA antibody immune complex.

H. Kitamura, K. Shindo, M. Sasabe, E. Matsuura, N. Sakairi, N. Nishi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Monoclonal antibodies were purified from the culture medium of hybridoma from systemic lupus erythematosus (SLE)-prone MRL/Mp-Ipr-Ipr mice with affinity chromatography. Binding activity of the antibodies to double-stranded DNA from salmon milt was actually shown in enzyme-linked immunosorbent assay (ELISA). Circular dichroism (CD) spectrum of DNA solution (150 mM NaCl, 20 mM Na2HPO4, pH 7.4) which showed B-type conformation, changed significantly by addition of the anti-DNA antibodies. These results indicate that conformational change of DNA occurred by binding of anti-DNA antibodies, forming DNA-anti-DNA antibody immune complex.

Original languageEnglish
Pages (from-to)145-146
Number of pages2
JournalNucleic acids symposium series
Issue number37
Publication statusPublished - 1997
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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