Abstract
Monoclonal antibodies were purified from the culture medium of hybridoma from systemic lupus erythematosus (SLE)-prone MRL/Mp-Ipr-Ipr mice with affinity chromatography. Binding activity of the antibodies to double-stranded DNA from salmon milt was actually shown in enzyme-linked immunosorbent assay (ELISA). Circular dichroism (CD) spectrum of DNA solution (150 mM NaCl, 20 mM Na2HPO4, pH 7.4) which showed B-type conformation, changed significantly by addition of the anti-DNA antibodies. These results indicate that conformational change of DNA occurred by binding of anti-DNA antibodies, forming DNA-anti-DNA antibody immune complex.
| Original language | English |
|---|---|
| Pages (from-to) | 145-146 |
| Number of pages | 2 |
| Journal | Nucleic acids symposium series |
| Issue number | 37 |
| Publication status | Published - 1997 |
| Externally published | Yes |
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Conformational change of DNA on the formation of DNA-anti-DNA antibody immune complex. / Kitamura, H.; Shindo, K.; Sasabe, M.; Matsuura, Eiji; Sakairi, N.; Nishi, N.
In: Nucleic acids symposium series, No. 37, 1997, p. 145-146.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Conformational change of DNA on the formation of DNA-anti-DNA antibody immune complex.
AU - Kitamura, H.
AU - Shindo, K.
AU - Sasabe, M.
AU - Matsuura, Eiji
AU - Sakairi, N.
AU - Nishi, N.
PY - 1997
Y1 - 1997
N2 - Monoclonal antibodies were purified from the culture medium of hybridoma from systemic lupus erythematosus (SLE)-prone MRL/Mp-Ipr-Ipr mice with affinity chromatography. Binding activity of the antibodies to double-stranded DNA from salmon milt was actually shown in enzyme-linked immunosorbent assay (ELISA). Circular dichroism (CD) spectrum of DNA solution (150 mM NaCl, 20 mM Na2HPO4, pH 7.4) which showed B-type conformation, changed significantly by addition of the anti-DNA antibodies. These results indicate that conformational change of DNA occurred by binding of anti-DNA antibodies, forming DNA-anti-DNA antibody immune complex.
AB - Monoclonal antibodies were purified from the culture medium of hybridoma from systemic lupus erythematosus (SLE)-prone MRL/Mp-Ipr-Ipr mice with affinity chromatography. Binding activity of the antibodies to double-stranded DNA from salmon milt was actually shown in enzyme-linked immunosorbent assay (ELISA). Circular dichroism (CD) spectrum of DNA solution (150 mM NaCl, 20 mM Na2HPO4, pH 7.4) which showed B-type conformation, changed significantly by addition of the anti-DNA antibodies. These results indicate that conformational change of DNA occurred by binding of anti-DNA antibodies, forming DNA-anti-DNA antibody immune complex.
UR - http://www.scopus.com/inward/record.url?scp=0031325947&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031325947&partnerID=8YFLogxK
M3 - Article
C2 - 9586041
AN - SCOPUS:0031325947
SP - 145
EP - 146
JO - Nucleic acids symposium series
JF - Nucleic acids symposium series
SN - 0261-3166
IS - 37
ER -