Confocal laser scanning microscopic observation of glycocalyx production by Staphylococcus aureus in skin lesions of bullous impetigo, atopic dermatitis and pemphigus foliaceus

H. Akiyama, T. Hamada, W. K. Huh, Osamu Yamasaki, Takashi Oono, W. Fujimoto, K. Iwatsuki

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Background: Glycocalyx collapses during dehydration to produce electron-dense accretions. Confocal laser scanning microscopy (CLSM) may be used to visualize fully hydrated microbial biofilms. Objectives: Using CLSM, to analyse glycocalyx production by Staphylococcus aureus cells in skin lesions of bullous impetigo, atopic dermatitis and pemphigus foliaceus. A second objective was to compare numbers of S. aureus cells in tissue sections prepared by different methods for routine light microscopy. Methods: S. aureus cells in skin lesions of impetigo, atopic dermatitis and pemphigus were stained with safranin, and positive staining with fluorescein isothiocyanate-conjugated concanavalin A was considered to indicate the presence of glycocalyx. Results: All S. aureus cells tested in skin lesions of impetigo, atopic dermatitis and pemphigus were covered with glycocalyx and formed microcolonies. The numbers of S. aureus cells in a routine light microscopy section were significantly lower than those in a frozen section that had not been dehydrated with ethanol. Conclusions: S. aureus cells generally produce glycocalyx in skin lesions of bullous impetigo, atopic dermatitis and pemphigus foliaceus, which accounts for the difficulty of removing S. aureus cells from these skin lesions. The glycocalyx may collapse during dehydration and most of the S. aureus cells may be carried away during preparation of routine light microscope sections.

Original languageEnglish
Pages (from-to)526-532
Number of pages7
JournalBritish Journal of Dermatology
Volume148
Issue number3
DOIs
Publication statusPublished - Mar 1 2003

Fingerprint

Impetigo
Glycocalyx
Pemphigus
Atopic Dermatitis
Blister
Staphylococcus aureus
Lasers
Skin
Dehydration
Light
Confocal Microscopy
Microscopy
Frozen Sections
Biofilms
Concanavalin A
Fluorescein
Ethanol
Electrons
Staining and Labeling

Keywords

  • Atopic dermatitis
  • Bullous impetigo
  • Confocal laser scanning microscopy
  • Frozen section
  • Glycocalyx
  • Pemphigus foliaceus
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Dermatology

Cite this

Confocal laser scanning microscopic observation of glycocalyx production by Staphylococcus aureus in skin lesions of bullous impetigo, atopic dermatitis and pemphigus foliaceus. / Akiyama, H.; Hamada, T.; Huh, W. K.; Yamasaki, Osamu; Oono, Takashi; Fujimoto, W.; Iwatsuki, K.

In: British Journal of Dermatology, Vol. 148, No. 3, 01.03.2003, p. 526-532.

Research output: Contribution to journalArticle

@article{f9a0bb65530049ff9a5eb96b052cbc7a,
title = "Confocal laser scanning microscopic observation of glycocalyx production by Staphylococcus aureus in skin lesions of bullous impetigo, atopic dermatitis and pemphigus foliaceus",
abstract = "Background: Glycocalyx collapses during dehydration to produce electron-dense accretions. Confocal laser scanning microscopy (CLSM) may be used to visualize fully hydrated microbial biofilms. Objectives: Using CLSM, to analyse glycocalyx production by Staphylococcus aureus cells in skin lesions of bullous impetigo, atopic dermatitis and pemphigus foliaceus. A second objective was to compare numbers of S. aureus cells in tissue sections prepared by different methods for routine light microscopy. Methods: S. aureus cells in skin lesions of impetigo, atopic dermatitis and pemphigus were stained with safranin, and positive staining with fluorescein isothiocyanate-conjugated concanavalin A was considered to indicate the presence of glycocalyx. Results: All S. aureus cells tested in skin lesions of impetigo, atopic dermatitis and pemphigus were covered with glycocalyx and formed microcolonies. The numbers of S. aureus cells in a routine light microscopy section were significantly lower than those in a frozen section that had not been dehydrated with ethanol. Conclusions: S. aureus cells generally produce glycocalyx in skin lesions of bullous impetigo, atopic dermatitis and pemphigus foliaceus, which accounts for the difficulty of removing S. aureus cells from these skin lesions. The glycocalyx may collapse during dehydration and most of the S. aureus cells may be carried away during preparation of routine light microscope sections.",
keywords = "Atopic dermatitis, Bullous impetigo, Confocal laser scanning microscopy, Frozen section, Glycocalyx, Pemphigus foliaceus, Staphylococcus aureus",
author = "H. Akiyama and T. Hamada and Huh, {W. K.} and Osamu Yamasaki and Takashi Oono and W. Fujimoto and K. Iwatsuki",
year = "2003",
month = "3",
day = "1",
doi = "10.1046/j.1365-2133.2003.05162.x",
language = "English",
volume = "148",
pages = "526--532",
journal = "British Journal of Dermatology",
issn = "0007-0963",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Confocal laser scanning microscopic observation of glycocalyx production by Staphylococcus aureus in skin lesions of bullous impetigo, atopic dermatitis and pemphigus foliaceus

AU - Akiyama, H.

AU - Hamada, T.

AU - Huh, W. K.

AU - Yamasaki, Osamu

AU - Oono, Takashi

AU - Fujimoto, W.

AU - Iwatsuki, K.

PY - 2003/3/1

Y1 - 2003/3/1

N2 - Background: Glycocalyx collapses during dehydration to produce electron-dense accretions. Confocal laser scanning microscopy (CLSM) may be used to visualize fully hydrated microbial biofilms. Objectives: Using CLSM, to analyse glycocalyx production by Staphylococcus aureus cells in skin lesions of bullous impetigo, atopic dermatitis and pemphigus foliaceus. A second objective was to compare numbers of S. aureus cells in tissue sections prepared by different methods for routine light microscopy. Methods: S. aureus cells in skin lesions of impetigo, atopic dermatitis and pemphigus were stained with safranin, and positive staining with fluorescein isothiocyanate-conjugated concanavalin A was considered to indicate the presence of glycocalyx. Results: All S. aureus cells tested in skin lesions of impetigo, atopic dermatitis and pemphigus were covered with glycocalyx and formed microcolonies. The numbers of S. aureus cells in a routine light microscopy section were significantly lower than those in a frozen section that had not been dehydrated with ethanol. Conclusions: S. aureus cells generally produce glycocalyx in skin lesions of bullous impetigo, atopic dermatitis and pemphigus foliaceus, which accounts for the difficulty of removing S. aureus cells from these skin lesions. The glycocalyx may collapse during dehydration and most of the S. aureus cells may be carried away during preparation of routine light microscope sections.

AB - Background: Glycocalyx collapses during dehydration to produce electron-dense accretions. Confocal laser scanning microscopy (CLSM) may be used to visualize fully hydrated microbial biofilms. Objectives: Using CLSM, to analyse glycocalyx production by Staphylococcus aureus cells in skin lesions of bullous impetigo, atopic dermatitis and pemphigus foliaceus. A second objective was to compare numbers of S. aureus cells in tissue sections prepared by different methods for routine light microscopy. Methods: S. aureus cells in skin lesions of impetigo, atopic dermatitis and pemphigus were stained with safranin, and positive staining with fluorescein isothiocyanate-conjugated concanavalin A was considered to indicate the presence of glycocalyx. Results: All S. aureus cells tested in skin lesions of impetigo, atopic dermatitis and pemphigus were covered with glycocalyx and formed microcolonies. The numbers of S. aureus cells in a routine light microscopy section were significantly lower than those in a frozen section that had not been dehydrated with ethanol. Conclusions: S. aureus cells generally produce glycocalyx in skin lesions of bullous impetigo, atopic dermatitis and pemphigus foliaceus, which accounts for the difficulty of removing S. aureus cells from these skin lesions. The glycocalyx may collapse during dehydration and most of the S. aureus cells may be carried away during preparation of routine light microscope sections.

KW - Atopic dermatitis

KW - Bullous impetigo

KW - Confocal laser scanning microscopy

KW - Frozen section

KW - Glycocalyx

KW - Pemphigus foliaceus

KW - Staphylococcus aureus

UR - http://www.scopus.com/inward/record.url?scp=0037362664&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037362664&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2133.2003.05162.x

DO - 10.1046/j.1365-2133.2003.05162.x

M3 - Article

C2 - 12653745

AN - SCOPUS:0037362664

VL - 148

SP - 526

EP - 532

JO - British Journal of Dermatology

JF - British Journal of Dermatology

SN - 0007-0963

IS - 3

ER -