Confocal laser scanning microscopic observation of glycocalyx production by Staphylococcus aureus in mouse skin: Does S. aureus generally produce a biofilm on damaged skin?

Hisanori Akiyama, W. K. Huh, Osamu Yamasaki, Takashi Oono, K. Iwatsuki

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Background: Bacteria that adhere to damaged tissues encase themselves in a hydrated matrix of polysaccharides, forming a slimy layer known as a biofilm. This is the first report of detection of glycocalyx production by Staphylococcus aureus using confocal laser scanning microscopy (CLSM) on damaged skin tissues. Objectives: To analyse glycocalyx production by S. aureus cells on damaged skin tissues and the influence of polymorphonuclear leucocytes (PMNs) and various antimicrobial agents on its production using CLSM in cyclophosphamide (Cy)-treated (neutropenic) or non-Cy-treated (normal) mice. Methods: S. aureus cells were inoculated on damaged skin tissues in neutropenic or normal mice with or without topical application of antimicrobial agents. S. aureus cells were stained with safranine, and positive staining with fluorescein isothiocyanate-conjugated concanavalin A was considered to indicate the presence of glycocalyx. Results: All S. aureus cells tested on damaged skin tissues formed microcolonies encircled by glycocalyx. The colony counts of S. aureus cells on croton oil dermatitis in normal mice treated with 2% fusidic acid ointment were about 100 times lower than those in neutropenic mice (control). Conclusions: As S. aureus cells can generally produce a biofilm on damaged skin tissues, antimicrobial agents may not eradicate S. aureus cells without the help of PMNs. S. aureus glycocalyx may play a crucial role in colonization and adherence to damaged skin tissues.

Original languageEnglish
Pages (from-to)879-885
Number of pages7
JournalBritish Journal of Dermatology
Volume147
Issue number5
DOIs
Publication statusPublished - 2002

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Glycocalyx
Biofilms
Staphylococcus aureus
Lasers
Skin
Anti-Infective Agents
Confocal Microscopy
Croton Oil
Fusidic Acid
Dermatitis
Concanavalin A
Ointments
Fluorescein
Cyclophosphamide
Polysaccharides
Neutrophils
Staining and Labeling
Bacteria

Keywords

  • Antimicrobial agent
  • Biofilm
  • Confocal laser scanning microscopy
  • Glycocalyx
  • Polymorphonuclear leucocyte
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Dermatology

Cite this

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title = "Confocal laser scanning microscopic observation of glycocalyx production by Staphylococcus aureus in mouse skin: Does S. aureus generally produce a biofilm on damaged skin?",
abstract = "Background: Bacteria that adhere to damaged tissues encase themselves in a hydrated matrix of polysaccharides, forming a slimy layer known as a biofilm. This is the first report of detection of glycocalyx production by Staphylococcus aureus using confocal laser scanning microscopy (CLSM) on damaged skin tissues. Objectives: To analyse glycocalyx production by S. aureus cells on damaged skin tissues and the influence of polymorphonuclear leucocytes (PMNs) and various antimicrobial agents on its production using CLSM in cyclophosphamide (Cy)-treated (neutropenic) or non-Cy-treated (normal) mice. Methods: S. aureus cells were inoculated on damaged skin tissues in neutropenic or normal mice with or without topical application of antimicrobial agents. S. aureus cells were stained with safranine, and positive staining with fluorescein isothiocyanate-conjugated concanavalin A was considered to indicate the presence of glycocalyx. Results: All S. aureus cells tested on damaged skin tissues formed microcolonies encircled by glycocalyx. The colony counts of S. aureus cells on croton oil dermatitis in normal mice treated with 2{\%} fusidic acid ointment were about 100 times lower than those in neutropenic mice (control). Conclusions: As S. aureus cells can generally produce a biofilm on damaged skin tissues, antimicrobial agents may not eradicate S. aureus cells without the help of PMNs. S. aureus glycocalyx may play a crucial role in colonization and adherence to damaged skin tissues.",
keywords = "Antimicrobial agent, Biofilm, Confocal laser scanning microscopy, Glycocalyx, Polymorphonuclear leucocyte, Staphylococcus aureus",
author = "Hisanori Akiyama and Huh, {W. K.} and Osamu Yamasaki and Takashi Oono and K. Iwatsuki",
year = "2002",
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language = "English",
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T1 - Confocal laser scanning microscopic observation of glycocalyx production by Staphylococcus aureus in mouse skin

T2 - Does S. aureus generally produce a biofilm on damaged skin?

AU - Akiyama, Hisanori

AU - Huh, W. K.

AU - Yamasaki, Osamu

AU - Oono, Takashi

AU - Iwatsuki, K.

PY - 2002

Y1 - 2002

N2 - Background: Bacteria that adhere to damaged tissues encase themselves in a hydrated matrix of polysaccharides, forming a slimy layer known as a biofilm. This is the first report of detection of glycocalyx production by Staphylococcus aureus using confocal laser scanning microscopy (CLSM) on damaged skin tissues. Objectives: To analyse glycocalyx production by S. aureus cells on damaged skin tissues and the influence of polymorphonuclear leucocytes (PMNs) and various antimicrobial agents on its production using CLSM in cyclophosphamide (Cy)-treated (neutropenic) or non-Cy-treated (normal) mice. Methods: S. aureus cells were inoculated on damaged skin tissues in neutropenic or normal mice with or without topical application of antimicrobial agents. S. aureus cells were stained with safranine, and positive staining with fluorescein isothiocyanate-conjugated concanavalin A was considered to indicate the presence of glycocalyx. Results: All S. aureus cells tested on damaged skin tissues formed microcolonies encircled by glycocalyx. The colony counts of S. aureus cells on croton oil dermatitis in normal mice treated with 2% fusidic acid ointment were about 100 times lower than those in neutropenic mice (control). Conclusions: As S. aureus cells can generally produce a biofilm on damaged skin tissues, antimicrobial agents may not eradicate S. aureus cells without the help of PMNs. S. aureus glycocalyx may play a crucial role in colonization and adherence to damaged skin tissues.

AB - Background: Bacteria that adhere to damaged tissues encase themselves in a hydrated matrix of polysaccharides, forming a slimy layer known as a biofilm. This is the first report of detection of glycocalyx production by Staphylococcus aureus using confocal laser scanning microscopy (CLSM) on damaged skin tissues. Objectives: To analyse glycocalyx production by S. aureus cells on damaged skin tissues and the influence of polymorphonuclear leucocytes (PMNs) and various antimicrobial agents on its production using CLSM in cyclophosphamide (Cy)-treated (neutropenic) or non-Cy-treated (normal) mice. Methods: S. aureus cells were inoculated on damaged skin tissues in neutropenic or normal mice with or without topical application of antimicrobial agents. S. aureus cells were stained with safranine, and positive staining with fluorescein isothiocyanate-conjugated concanavalin A was considered to indicate the presence of glycocalyx. Results: All S. aureus cells tested on damaged skin tissues formed microcolonies encircled by glycocalyx. The colony counts of S. aureus cells on croton oil dermatitis in normal mice treated with 2% fusidic acid ointment were about 100 times lower than those in neutropenic mice (control). Conclusions: As S. aureus cells can generally produce a biofilm on damaged skin tissues, antimicrobial agents may not eradicate S. aureus cells without the help of PMNs. S. aureus glycocalyx may play a crucial role in colonization and adherence to damaged skin tissues.

KW - Antimicrobial agent

KW - Biofilm

KW - Confocal laser scanning microscopy

KW - Glycocalyx

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KW - Staphylococcus aureus

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