Configuration of Ten Light-Harvesting Chlorophyll a/b Complex I Subunits in Chlamydomonas reinhardtii Photosystem I

Shinichiro Ozawa, Till Bald, Takahito Onishi, Huidan Xue, Takunori Matsumura, Ryota Kubo, Hiroko Takahashi, Michael Hippler, Yuichiro Takahashi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In plants, the photosystem I (PSI) core complex stably associates with its light-harvesting chlorophyll a/b complex I (LHCI) to form the PSI-LHCI supercomplex. The vascular plant PSI core complex associates with four distinct LHCI subunits, whereas that of the green alga Chlamydomonas reinhardtii binds nine distinct LHCI subunits (LHCA1-LHCA9). The stoichiometry and configuration of these LHCI subunits in the PSI-LHCI supercomplex of C. reinhardtii remain controversial. Here, we determined the stoichiometry of the nine distinct LHCI subunits relative to PSI subunits through uniform labeling of total proteins using 14C. We separated the nine LHCI polypeptides by three different sodium dodecyl sulfate-polyacrylamide gel electrophoresis systems. Our data revealed that the PSI-LHCI supercomplex contains two LHCA1 proteins and one of each of the other eight LHCI subunits. Subsequently, we identified their cross-linked products by immunodetection and mass spectrometry to determine the configuration of the 10 LHCI subunits within the PSI-LHCI supercomplex. Furthermore, analyses of PSI-LHCI complexes isolated from ΔLHCA2 and ΔLHCA5 mutants and oligomeric LHCI from a PSI-deficient (ΔpsaA/B) mutant provided supporting evidence for the LHCI subunit configuration. In conclusion, eight LHCI subunits bind to the PSI core at the site of PSAF subunit in two layers: LHCA1-LHCA8-LHCA7-LHCA3 from PSAG to PSAK, in the inner layer, and LHCA1-LHCA4-LHCA6-LHCA5 in the outer layer. The other two LHCI subunits, LHCA2 and LHCA9, bind PSAB between PSAG and PSAH, PSAG-LHCA9-LHCA2-PSAH. Our study provides new insights into the LHCI configuration linked to the PSI core.

Original languageEnglish
Pages (from-to)583-595
Number of pages13
JournalPlant Physiology
Volume178
Issue number2
DOIs
Publication statusPublished - Oct 1 2018

Fingerprint

Photosystem I Protein Complex
Chlamydomonas reinhardtii
photosystem I
chlorophyll
Light
stoichiometry
chlorophyll b
chlorophyll a
mutants
Chlorophyta
vascular plants
Sodium Dodecyl Sulfate
polyacrylamide gel electrophoresis
Blood Vessels
Polyacrylamide Gel Electrophoresis
polypeptides
Mass Spectrometry
Proteins
proteins

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Configuration of Ten Light-Harvesting Chlorophyll a/b Complex I Subunits in Chlamydomonas reinhardtii Photosystem I. / Ozawa, Shinichiro; Bald, Till; Onishi, Takahito; Xue, Huidan; Matsumura, Takunori; Kubo, Ryota; Takahashi, Hiroko; Hippler, Michael; Takahashi, Yuichiro.

In: Plant Physiology, Vol. 178, No. 2, 01.10.2018, p. 583-595.

Research output: Contribution to journalArticle

Ozawa, Shinichiro ; Bald, Till ; Onishi, Takahito ; Xue, Huidan ; Matsumura, Takunori ; Kubo, Ryota ; Takahashi, Hiroko ; Hippler, Michael ; Takahashi, Yuichiro. / Configuration of Ten Light-Harvesting Chlorophyll a/b Complex I Subunits in Chlamydomonas reinhardtii Photosystem I. In: Plant Physiology. 2018 ; Vol. 178, No. 2. pp. 583-595.
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