@article{e48c83e509dd4036923cf06aff442644,
title = "Conditional transformation of immunoglobulin mutation pattern from gene conversion into point mutation by controlling XRCC3 expression in the DT40 B cell line",
abstract = "A hypermutating B cell line DT40 is useful for screening antibodies and improving affinity of the selected antibodies in vitro. To perform affinity maturation efficiently, we generated an engineered DT40 line whose immunoglobulin mutation pattern can be transformed from gene conversion into point mutation by conditional suppression of XRCC3 expression.",
keywords = "Chicken B cell line, Gene conversion, Monoclonal antibody, Point mutation, XRCC3",
author = "Masamichi Kajita and Masaki Magari and Kagefumi Todo and Naoki Kanayama and Hitoshi Ohmori",
note = "Funding Information: We thank Dr. H. Arakawa (GSF National Research Center for Environment and Health, Neuherverg-Munich, Germany) for the loxP-flanked gpt and bsr genes, and Dr. M. Takata (Kyoto University, Kyoto, Japan) for gene constructs and related information of the cXRCC3 gene. This work was supported in part by Grants from Japan Science and Technology Agency (JST) and from New Energy and Industrial Technology Development Organization (NEDO) . Copyright: Copyright 2010 Elsevier B.V., All rights reserved.",
year = "2010",
month = apr,
doi = "10.1016/j.jbiosc.2009.09.050",
language = "English",
volume = "109",
pages = "407--410",
journal = "Journal of Bioscience and Bioengineering",
issn = "1389-1723",
publisher = "The Society for Biotechnology, Japan",
number = "4",
}