Abstract
A hypermutating B cell line DT40 is useful for screening antibodies and improving affinity of the selected antibodies in vitro. To perform affinity maturation efficiently, we generated an engineered DT40 line whose immunoglobulin mutation pattern can be transformed from gene conversion into point mutation by conditional suppression of XRCC3 expression.
| Original language | English |
|---|---|
| Pages (from-to) | 407-410 |
| Number of pages | 4 |
| Journal | Journal of Bioscience and Bioengineering |
| Volume | 109 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Apr 1 2010 |
Keywords
- Chicken B cell line
- Gene conversion
- Monoclonal antibody
- Point mutation
- XRCC3
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
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Kajita, M., Magari, M., Todo, K., Kanayama, N., & Ohmori, H. (2010). Conditional transformation of immunoglobulin mutation pattern from gene conversion into point mutation by controlling XRCC3 expression in the DT40 B cell line. Journal of Bioscience and Bioengineering, 109(4), 407-410. https://doi.org/10.1016/j.jbiosc.2009.09.050