Conditional transformation of immunoglobulin mutation pattern from gene conversion into point mutation by controlling XRCC3 expression in the DT40 B cell line

Masamichi Kajita; Masaki Magari; Kagefumi Todo; Naoki Kanayama; Hitoshi Ohmori

doi:10.1016/j.jbiosc.2009.09.050

Conditional transformation of immunoglobulin mutation pattern from gene conversion into point mutation by controlling XRCC3 expression in the DT40 B cell line

Masamichi Kajita, Masaki Magari, Kagefumi Todo, Naoki Kanayama, Hitoshi Ohmori

Research output: Contribution to journal › Article

7 Citations (Scopus)

Abstract

A hypermutating B cell line DT40 is useful for screening antibodies and improving affinity of the selected antibodies in vitro. To perform affinity maturation efficiently, we generated an engineered DT40 line whose immunoglobulin mutation pattern can be transformed from gene conversion into point mutation by conditional suppression of XRCC3 expression.

Original language	English
Pages (from-to)	407-410
Number of pages	4
Journal	Journal of Bioscience and Bioengineering
Volume	109
Issue number	4
DOIs	https://doi.org/10.1016/j.jbiosc.2009.09.050
Publication status	Published - Apr 1 2010

Fingerprint

Keywords

Chicken B cell line
Gene conversion
Monoclonal antibody
Point mutation
XRCC3

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

Cite this

Kajita, M., Magari, M., Todo, K., Kanayama, N., & Ohmori, H. (2010). Conditional transformation of immunoglobulin mutation pattern from gene conversion into point mutation by controlling XRCC3 expression in the DT40 B cell line. Journal of Bioscience and Bioengineering, 109(4), 407-410. https://doi.org/10.1016/j.jbiosc.2009.09.050

Conditional transformation of immunoglobulin mutation pattern from gene conversion into point mutation by controlling XRCC3 expression in the DT40 B cell line. / Kajita, Masamichi; Magari, Masaki; Todo, Kagefumi; Kanayama, Naoki; Ohmori, Hitoshi.

In: Journal of Bioscience and Bioengineering, Vol. 109, No. 4, 01.04.2010, p. 407-410.

Research output: Contribution to journal › Article

@article{e48c83e509dd4036923cf06aff442644,

title = "Conditional transformation of immunoglobulin mutation pattern from gene conversion into point mutation by controlling XRCC3 expression in the DT40 B cell line",

abstract = "A hypermutating B cell line DT40 is useful for screening antibodies and improving affinity of the selected antibodies in vitro. To perform affinity maturation efficiently, we generated an engineered DT40 line whose immunoglobulin mutation pattern can be transformed from gene conversion into point mutation by conditional suppression of XRCC3 expression.",

keywords = "Chicken B cell line, Gene conversion, Monoclonal antibody, Point mutation, XRCC3",

author = "Masamichi Kajita and Masaki Magari and Kagefumi Todo and Naoki Kanayama and Hitoshi Ohmori",

year = "2010",

month = apr

day = "1",

doi = "10.1016/j.jbiosc.2009.09.050",

language = "English",

volume = "109",

pages = "407--410",

journal = "Journal of Bioscience and Bioengineering",

issn = "1389-1723",

publisher = "The Society for Biotechnology, Japan",

number = "4",

}

TY - JOUR

T1 - Conditional transformation of immunoglobulin mutation pattern from gene conversion into point mutation by controlling XRCC3 expression in the DT40 B cell line

AU - Kajita, Masamichi

AU - Magari, Masaki

AU - Todo, Kagefumi

AU - Kanayama, Naoki

AU - Ohmori, Hitoshi

PY - 2010/4/1

Y1 - 2010/4/1

N2 - A hypermutating B cell line DT40 is useful for screening antibodies and improving affinity of the selected antibodies in vitro. To perform affinity maturation efficiently, we generated an engineered DT40 line whose immunoglobulin mutation pattern can be transformed from gene conversion into point mutation by conditional suppression of XRCC3 expression.

AB - A hypermutating B cell line DT40 is useful for screening antibodies and improving affinity of the selected antibodies in vitro. To perform affinity maturation efficiently, we generated an engineered DT40 line whose immunoglobulin mutation pattern can be transformed from gene conversion into point mutation by conditional suppression of XRCC3 expression.

KW - Chicken B cell line

KW - Gene conversion

KW - Monoclonal antibody

KW - Point mutation

KW - XRCC3

UR - http://www.scopus.com/inward/record.url?scp=77649257039&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77649257039&partnerID=8YFLogxK

U2 - 10.1016/j.jbiosc.2009.09.050

DO - 10.1016/j.jbiosc.2009.09.050

M3 - Article

C2 - 20226386

AN - SCOPUS:77649257039

VL - 109

SP - 407

EP - 410

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

SN - 1389-1723

IS - 4

ER -

Access to Document

10.1016/j.jbiosc.2009.09.050