Conditional transformation of immunoglobulin mutation pattern from gene conversion into point mutation by controlling XRCC3 expression in the DT40 B cell line

Masamichi Kajita, Masaki Magari, Kagefumi Todo, Naoki Kanayama, Hitoshi Ohmori

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A hypermutating B cell line DT40 is useful for screening antibodies and improving affinity of the selected antibodies in vitro. To perform affinity maturation efficiently, we generated an engineered DT40 line whose immunoglobulin mutation pattern can be transformed from gene conversion into point mutation by conditional suppression of XRCC3 expression.

Original languageEnglish
Pages (from-to)407-410
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume109
Issue number4
DOIs
Publication statusPublished - Apr 1 2010

Keywords

  • Chicken B cell line
  • Gene conversion
  • Monoclonal antibody
  • Point mutation
  • XRCC3

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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