Conditional transformation of immunoglobulin mutation pattern from gene conversion into point mutation by controlling XRCC3 expression in the DT40 B cell line

Masamichi Kajita; Masaki Magari; Kagefumi Todo; Naoki Kanayama; Hitoshi Ohmori

doi:10.1016/j.jbiosc.2009.09.050

Conditional transformation of immunoglobulin mutation pattern from gene conversion into point mutation by controlling XRCC3 expression in the DT40 B cell line

Masamichi Kajita, Masaki Magari, Kagefumi Todo, Naoki Kanayama, Hitoshi Ohmori

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

A hypermutating B cell line DT40 is useful for screening antibodies and improving affinity of the selected antibodies in vitro. To perform affinity maturation efficiently, we generated an engineered DT40 line whose immunoglobulin mutation pattern can be transformed from gene conversion into point mutation by conditional suppression of XRCC3 expression.

Original language	English
Pages (from-to)	407-410
Number of pages	4
Journal	Journal of Bioscience and Bioengineering
Volume	109
Issue number	4
DOIs	https://doi.org/10.1016/j.jbiosc.2009.09.050
Publication status	Published - Apr 1 2010

Keywords

Chicken B cell line
Gene conversion
Monoclonal antibody
Point mutation
XRCC3

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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Conditional transformation of immunoglobulin mutation pattern from gene conversion into point mutation by controlling XRCC3 expression in the DT40 B cell line. / Kajita, Masamichi; Magari, Masaki; Todo, Kagefumi; Kanayama, Naoki; Ohmori, Hitoshi.

In: Journal of Bioscience and Bioengineering, Vol. 109, No. 4, 01.04.2010, p. 407-410.

Research output: Contribution to journal › Article › peer-review

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AU - Ohmori, Hitoshi

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